FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
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Reference
Citation
Brogan, D., Akbari, O. (2019.12.8). CasRx-specific guide RNA constructs and insertions. 
FlyBase ID
FBrf0244315
Publication Type
Personal communication to FlyBase
Abstract
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Text of Personal Communication 
The following information accompanied stocks donated to the Bloomington Stock Center by Dan Brogan and Omar Akbari, University of California at San Diego.
P{U6-wg.RxgRNA} expresses a CasRx-specific guide RNA directed against wg ubiquitously under the control of  snRNA:U6:96Ac  regulatory sequences. The construct also contains a mini-white marker.
P{U6-GFP.RxgRNA,OpIE2-GFP} expresses a CasRx-specific guide RNA directed against GFP ubiquitously under the control of  snRNA:U6:96Ac  regulatory sequences. It also expresses GFP ubiquitously under the control of OpIE2 sequences. The construct also contains a mini-white marker.
P{U6-wg.RxgRNA}attP2 and P{U6-GFP.RxgRNA,OpIE2-GFP}attP2 are insertions into the P{CaryP}attP2 target site at 68A4,  3L:11070538  (R6).
DOI
Related Publication(s)
Research paper

Programmable RNA Targeting Using CasRx in Flies.
Buchman et al., 2020, CRISPR J 3(3): 164--176 [FBrf0246003]

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    Language of Publication
    English
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    Data From Reference
    Alleles (4)
    Genes (3)
    Natural transposons (1)
    Insertions (3)
    Experimental Tools (1)
    Transgenic Constructs (2)