The following information accompanied stocks donated to the Bloomington Stock Center by Vince Archambault, Universite de Montreal.
P{UASp-polo.db.MYC} is identical to P{UASp-polo.MYC} except for sequence changes causing R309A and L312A amino acid substitutions inactivating the destruction box that render polo resistant to proteasomal degradation.
P{UASp-polo.HK.MYC} is identical to P{UASp-polo.MYC} except for sequence changes altering the Polo-Box Domain with H518A and K520A substitutions.
P{UASp-polo.KD.MYC} is identical to P{UASp-polo.MYC} except for sequence changes disrupting the kinase domain with a K54M substitution.
P{UASp-polo.9A.MYC} is identical to P{UASp-polo.MYC} except for sequence changes resulting in the replacement of nine phosphorylatable serines or threonines in the linker region with alanines (S299A, T302A, S306A, T323A, S327A, T328A, S348A, S353A and S357A).
P{UASp-polo.9D.MYC} is identical to P{UASp-polo.MYC} except for sequence changes resulting in the replacement of nine phosphorylatable serines or threonines in the linker region with phosphomimetic aspartic acid residues (S299D, T302D, S306D, T323D, S327D, T328D, S348D, S353D and S357D).
P{UASp-PrA.polo} expresses polo with a Protein A tag at its N terminus under UASp control. The vector and methods used to generate the construct are described in Lipinszki et al. (2014) "Affinity purification of protein complexes from Drosophila embryos in cell cycle studies" Methods Mol. Biol. 1170: 571-588 (FBrf0225277).
P{UASp-PrA.polo.PBDmut} is identical to P{UASp-PrA.polo} except for sequence changes altering the Polo-Box Domain with W395F, H518A and K520A substitutions.
P{UASp-polo.T182A.PrA} expresses polo with a Protein A tag at its C terminus under UASp control. It has sequence changes resulting in substitution of alanine for threonine at residue 182 resulting in the elimination of a phosphorylation site.
P{UASp-polo.T182D.PrA} expresses polo with a Protein A tag at its C terminus under UASp control. It has sequence changes resulting in substitution of aspartic acid for threonine at residue 182 resulting in the presence of a phosphomimetic amino acid.
The following insertions lie on the second chromosome:
P{UASp-polo.MYC}2
P{UASp-polo.T182A.MYC}2
P{UASp-polo.T182D.MYC}2
P{UASp-polo.db.MYC}2
P{UASp-polo.HK.MYC}2
P{UASp-polo.KD.MYC}2
P{UASp-polo.9D.MYC}2
P{UASp-PrA.polo}2
P{UASp-PrA.polo.PBDmut}2
P{UASp-polo.T182A.PrA}2
P{UASp-polo.T182D.PrA}2
The following insertions lie on the third chromosome:
P{UASp-polo.MYC}3
P{UASp-polo.T182A.MYC}3
P{UASp-polo.T182D.MYC}3
P{UASp-polo.db.MYC}3
P{UASp-polo.HK.MYC}3
P{UASp-polo.9A.MYC}3
P{UASp-PrA.polo}3
P{UASp-PrA.polo.PBDmut}3
P{UASp-polo.T182A.PrA}3
P{UASp-polo.T182D.PrA}3