FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
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Holder, M., Tapon, N., Ribeiro, P.S. (2019.12.20). Location data for CG7094[F2] and gish[17]. 
FlyBase ID
FBrf0244455
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Personal communication to FlyBase
Abstract
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Text of Personal Communication 
gish17
gish17 gRNA target: AATGGAGCCTATGAAGTCAA-AGG
The gish17 deletion removes 5bp from exon "FBtr0100332-E3".
Exon sequence in gish17 allele (hyphen marks position of deletion):
GCAAAAACCTTTACAACAATGAACATGTAGCAATAAAAATGGAGCCTATGAA-AGGCTCCGCAACTACACTTAGAGTATAGGTTTTATAAACTATTAGGATCACATG
wt:       AATGGAGCCTATGAAGTCAAAGGCTCCGCA
gish17 : AATGGAGCCTATGAA*****AGGCTCCGCA
This deletion creates a frameshift and a premature termination codon after 8 aa of nonsense.
Predicted translation:
MQRRERQASGGGPQTTTAGPSAGNVANATTALAGGKSSSNNMYSTRQSVSTTTGVLMVGPNFRVGKKIGCGNFGELRLGKNLYNNEHVAIKMEPMK-GSATTLRV-
CG7094F2
CG7094F2 gRNAs
Upstream target site ATATCTCGGACTAAGCATCA-CGG
Downstream target site ACGGGGTTGTGAGCCTCAGC-GGG
Genomic sequence surrounding the CG7094F2 deletion:
TGGTCAGAATGTTAAAACGCCCACTATAACATCCGCTCATAATTTGTCACGCCAACACATTTAAAAAATGTTTGATTTTAAATTTTCCTGCCAAACTGTATTCGTTCTGTCGTTATTTTCTGGTACTTTTTTAAGTGTGGCTTTTACTATTTAGTCAGAGTGGCTAGATAAAATATCGAACAGCAAATTTTGTCCAAGGCAGTAAAGTCACAAAATGCCTTAAATGTCAAAATCGTAGTACAGTTCTCAAAGAAAATTAATAAAGATAAGTCTCACAGGGCTCCAAAAATACTTCAGAGAAGACATTGAGGCGAGAATCTGAAAGCCATAACAATGGCAAAACAAGGAGCAACAAATGGGAAAGTTCGTAATGCATCTCTTCATCTGGAGAAGCTGTTGATCGGGGGAAAATATCGGCTGGTAAAGCCCATTGGGTCCGGGTCCTTCGGCGATATATATCTCGGACTAAGCA-GCGGGACAAGGAGCGGCACAAGGATTTGGAGCTGGATCGACTGCACAAAACTTCCACCCAACAGGCAAAGTGCTCTAATGGACATCTAACAAACCGATATGATCGCATTGGCGATGGAGGCATTAGCAAGCGAAAGTAACATATAACAAAATTATTTCCATTTTAAAGTTTACTACTTCATGTTGGAGTGGTTTTATTTCGCAAATAAATCTTTTAAAAACCCCACTTGTAGTGGTTTTATTTTGGTTTATTTCCAAAATTGAACCTTTCCCCATTTCAGGTATTTTTTCAATAAATTACAATTAAGTATTGAATGGTCTCAGCACTTTATACTTGTCATGACCAAACTTTTTTATTTCGCTACATCTT
There has been an 895 bp deletion between TATCTCGGACTAAGCA and GCGGGACAAGGAGC (position marked with a hyphen above).
Predicted translation:
MAKQGATNGKVRNASLHLEKLLIGGKYRLVKPIGSGSFGDIYLGLS-SGTRSGTRIWSWIDCTKLPPNRQSALMDI-
The predicted protein would consist of 46aa of normal CG7094 sequence (including an E5G SNP which is present in our in-house nosCas9 stock), then 29 aa of nonsense (75 aa total length).
The whole kinase domain has been deleted.
DOI
Related Publication(s)
Research paper

Casein kinase 1 family proteins promote Slimb-dependent Expanded degradation.
Fulford et al., 2019, eLife 8: e46592 [FBrf0243590]

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