The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Jianzhi Zeng and Yulong Li, Peking University, China. The following transgenes each contain the GRAB(GACh3.0) acetylcholine sensor described in Jing et al., 2020, Nat. Methods 17(11): 1139--1146 <up>FBrf0247870</up>. P{10XUAS-GACh3.0} and PBac{10XUAS-GACh3.0} contain GRAB(GACh3.0) expressed under the control of UAS sequences. P{10XlexAop-GACh3.0} and PBac{10XlexAop-GACh3.0} contain GRAB(GACh3.0) expressed under the control of lexAop sequences. P{10XUAS-GACh3.0}attP40 is an insertion on the second chromosome in an attP site at 25C6, 2L:5108448 (r6). PBac{10XUAS-GACh3.0}VK00005 is an insertion on the third chromosome in an attP site at 75A10, 3L:17952108 (r6). P{10XlexAop-GACh3.0}attP40 is an insertion on the second chromosome in an attP site at 25C6, 2L:5108448 (r6). PBac{10XlexAop-GACh3.0}VK00005 is an insertion on the third chromosome in an attP site at 75A10, 3L:17952108 (r6).