FlyBase Reference Report: Looger, 2020.6.1, 20XUAS-iAChSnFR constructs and insertions from Loren Looger.

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Looger, L. (2020.6.1). 20XUAS-iAChSnFR constructs and insertions from Loren Looger.

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FBrf0245926

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Personal communication to FlyBase

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The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Loren Looger, Janelia Research Campus.
P{20XUAS-iAChSnFR} and PBac{20XUAS-iAChSnFR} carry the acetylcholine sensor, iAChSnFR, under the control of UAS. iAChSnFR is fusion protein based on the choline-binding protein opuBC from the hyperthermophilic Thermoanaerobacter sp. X513, with a circularly permutated form of sfGFP inserted. It has been optimized for increased specificity and affinity for acetylcholine.
P{20XUAS-iAChSnFR}attP18 is a homozygous and hemizygous viable and fertile insertion on the X chromosome.
P{20XUAS-iAChSnFR}attP40 is a homozygous viable and fertile insertion on the second chromosome.
PBac{20XUAS-iAChSnFR}VK00005 is a homozygous viable and fertile insertion on the third chromosome.

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English

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