FB2026_03 , released September 17, 2026
Reference Report
Open Close
Reference
Citation
Lois, C. (2020.8.27). intMEMOIR construct and insertion from Carlos Lois. 
FlyBase ID
FBrf0246654
Publication Type
Personal communication to FlyBase
Abstract
PubMed ID
PubMed Central ID
Text of Personal Communication 
The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Carlos Lois, California Institute of Technology.
P{UAS-Cerulean.intMEMOIR} (described in Chow et al., 2021, Science 372(6538): eabb3099, FBrf0248662) is composed of Cerulean plus 10 unique barcodes, each one of which is flanked by unique attP/attB sites recognized by Bxb1 integrase, all under the control of UAS. In the presence of  Bxb1:int , integrase activity can result in no change or stochastic deletion or inversion of individual barcodes.  Readout of each barcode status via sequential rounds of single molecule FISH allows the generation of lineage trees.
P{UAS-Cerulean.intMEMOIR}attP2  is an insertion on the third chromosome into attP2 (FBti0040535).
DOI
Related Publication(s)
Research paper

Imaging cell lineage with a synthetic digital recording system.
Chow et al., 2021, Science 372(6538): eabb3099 [FBrf0248662]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Abbreviation
    Title
    ISBN/ISSN
    Data From Reference
    Natural transposons (1)
    Insertions (2)
    Experimental Tools (4)
    Transgenic Constructs (1)