FB2026_03 , released September 17, 2026
Reference Report
Open Close
Reference
Citation
Kandul, N., Akbari, O. (2020.10.26). Kandul constructs and insertions. 
FlyBase ID
FBrf0247006
Publication Type
Personal communication to FlyBase
Abstract
PubMed ID
PubMed Central ID
Text of Personal Communication 
The following information accompanied stocks donated to the Bloomington Stock Center by Nikolay Kandul and Omar Akbari, University of California at San Diego.
P{U6-PolG2.gRNA.1} expresses a guide RNA directed against PolG2 ubiquitously under the control of  snRNA:U6:96Ac  regulatory sequences.
P{U6-PolG2.gRNA.1}attP1 is an insertion into P{CaryP}attP1 at 55C4,  2R:18357321 .
PBac{betaTub85D-Cas9.T2A.GFP}, PBac{Rcd-1r-Cas9.T2A.GFP} and PBac{exu-Cas9.T2A.GFP} express Cas9.T2A.GFP under control of betaTub85D, Rcd-1r and exu regulatory sequences. They are similar in structure to the previously described PBac{nos-Cas9.T2A.GFP}, PBac{Ubi-Cas9.T2A.GFP} and PBac{vas-Cas9.T2A.GFP} constructs.
PBac{exu-Cas9.T2A.GFP}VK00033, PBac{betaTub85D-Cas9.T2A.GFP}VK00033 and PBac{Rcd-1r-Cas9.T2A.GFP}VK00033 are insertions into PBac{y+-attP-3B}VK00033 at 65B2,  3L:6442676 .
DOI
Related Publication(s)
Research paper

A confinable home-and-rescue gene drive for population modification.
Kandul et al., 2021, eLife 10: e65939 [FBrf0248494]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Abbreviation
    Title
    ISBN/ISSN
    Data From Reference