FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
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Potter, C. (2021.5.13). Chemosensory receptor insertions from Chris Potter. 
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FBrf0249066
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The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Christopher Potter, Johns Hopkins University School of Medicine.
In the insertions below, Potter lab used a HACK knock-in approach to insert T2A-QF2 and lox-P-flanked 3xP3-mCherry at the ends of the indicated chemosensory receptor genes.  The end result is the expression of the full length gene plus a T2A linker and QF2 (resulting in both the receptor and QF2 as separate proteins in the pattern of the receptor). See Task et al., 2022, eLife 11: e72599 (FBrf0253272).
TI{2A-QF2}Ir25a2A-QF2 – T2A-QF2 and lox-P-flanked 3xP3-mCherry is inserted at the end of Ir25a
TI{2A-QF2}Ir25a2A-QF2.mCh- – T2A-QF2 is inserted at the end of Ir25a (the 3xP3-mCherry has been removed via Cre recombinase)
TI{2A-QF2}Ir76b2A-QF2 – T2A-QF2 and lox-P-flanked 3xP3-mCherry is inserted at the end of Ir76b
TI{2A-QF2}Ir8a2A-QF2 – T2A-QF2 and lox-P-flanked 3xP3-mCherry is inserted at the end of Ir8a
TI{2A-QF2}Orco2A-QF2 – T2A-QF2 and lox-P-flanked 3xP3-mCherry is inserted at the end of Orco
TI{2A-QF2}Orco2A-QF2.mCh- – T2A-QF2 is inserted at the end of Orco (the 3xP3-mCherry has been removed via Cre recombinase)
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Related Publication(s)
Research paper

Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons.
Task et al., 2022, eLife 11: e72599 [FBrf0253272]

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