The UAS-Notch transgenes we made in FBrf0252210 are not HA tagged - they were subcloned into the pUASg-HA.attB plasmid, but since the ORF contained a stop codon, the translation will stop at its endogenous termination site and the 3xHA tag will not be added to the protein. /reports/FBal0371151.html /reports/FBal0371152.html /reports/FBal0371153.html /reports/FBal0371154.html