The following information accompanied stocks donated to the Bloomington Stock Center by Mark Peifer, University of North Carolina.
TI{TI}cnoΔΔ is a knockout of cno that contains an attP site for future re-integration of sequence into the locus.
TI{TI}cnoΔΔ was generated in a two-step process from the TI{TI}cnoΔRA chromosome described in Perez-Vale et al., 2021, J. Cell Biol. 220(12): e202104087 (FBrf0251832). First, the loxP cassette in TI{TI}cnoΔRA was excised using cre recombinase, leaving a single attP site and a single loxP site in place of cno exons 2-6. Secondly, homology-directed repair was used to delete most of the remaining cno sequence (all exons downstream of exon 7 have been removed) and replace this deleted sequence with a loxP cassette containing a Disc\RFPDsRed(T1).3xP3.cHa marker.