The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Leo Pallanck, University of Washington.
P{UAS-PolG1.D185A.D268A} expresses PolG1 with amino acid changes D185A and D263A under the control of UAS. The two changes should eliminate the proofreading activity of the polymerase resulting in increased mutations in the mitochondrial genome.
P{UAS-PolG1.D185A.D268A}3 is a homozygous viable and fertile insertion on the third chromosome.