Abstract
DNA damage response (DDR) pathways in keto-enol genotoxicity have not been characterized, and few studies have reported genotoxic effects in non-target organisms. The present study shows that concentrations of 11.2, 22.4, 37.3 mg/L of Movento[®] 240SC and 12.3, 24.6, 41.1 mg/L of Envidor[®] 240SC for 72 h oral exposure induced DSBs by significantly increasing the percentage of γH2AV expression in regions 2b and 3 from the germarium of wild type females of Drosophila melanogaster Oregon R, compared to the control group (0.0 mg/L of insecticides), via confocal immunofluorescence microscopy. The comparison between both insecticides' reveals that only the Envidor[®] 240SC induces concentration-dependent DNA damage, as well as structural changes in the germarium. We determined that the DDR induced by Movento[®] 240SC depends on the activation of the ATM[tefu], Chk1[grp] and Chk2[lok] kinases by significantly increasing the percentage of expression of γH2AV in regions 2b and 3 of the germarium, and that ATR[mei-29D] and p53[dp53] kinases only respond at the highest concentration of 37.3 mg/L of Movento[®] 240SC. With the Envidor[®] 240SC insecticide, we determined that the DDR depends on the activation of the ATR[mei-29D]/Chk1[grp] and ATM[tefu]/Chk2[lok] kinases, and p53[dp53] by significantly increasing the percentage of expression of γH2AV in the germarium.
