FB2026_02 , released June 18, 2026
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Kogenaru, M. (2024.2.15). Update of details for UAS-hid construct described in FBrf0257558. 
FlyBase ID
FBrf0258880
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Personal communication to FlyBase
Abstract
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The P{UAS-hid.K} construct described in FBrf0257558 carries UAS upstream of an sfxa109-flanked cassette carrying hid coding sequences followed by mCherry coding sequences in reverse. 'sfxa109' is the target site for Rci recombinase (see PMID:12054795). In the absence of Rci recombinase, only hid protein is made. In the presence of Rci, the cassette can invert resulting in mCherry expression instead of hid.  Can be used to test for the presence of Rci recombinase.
FlyBase curator comment: the construct symbol has been renamed from P{UAS-hid.K} to P{UAS-sfxa109(hid.mCh-rev)} reflect the updated details.
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Related Publication(s)
Research paper

A drug stabilizable GAL80[ds] for conditional control of gene expression via GAL4-UAS and CRISPR-Cas9 systems in Drosophila.
Kogenaru et al., 2024, Sci. Rep. 14(1): 5893 [FBrf0259011]

Personal communication to FlyBase

P{UAS-hid.K} construct and insertion from M. Kogenaru.
Kogenaru, 2023.9.7, P{UAS-hid.K} construct and insertion from M. Kogenaru. [FBrf0257558]

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    English
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