Castro, K., Muradyan, V., Flota, P., Guanzon, J., Poole, N., Urrutia, H., Eivers, E. (2024). Drosophila Smad2 degradation occurs independently of linker phosphorylations. MicroPubl Biol 2024(): .
FlyBase ID
FBrf0259244
Publication Type
Research paper
Abstract
TGF-β signals are important for proliferation, differentiation, and cell fate determination during embryonic development and tissue homeostasis in adults. Drosophila Activin/TGF-β signals are transduced intracellularly when its transcription factor dSmad2 (also called Smad on X or Smox) is C-terminally phosphorylated by pathway receptors. Recently, it has been shown that receptor-activated dSmad2 undergoes bulk degradation, however, the mechanism of how this occurs is unknown. Here we investigated if two putative linker phosphorylation sites are involved in dSmad2 degradation. We demonstrate that degradation of activated-dSmad2 occurs independently of threonine phosphorylation at linker sites 252 and 277. We also show that dSmad2 degradation is not carried out by cellular proteasomes.
