FlyBase Reference Report: Geyer, 2024.11.19, P{UASp-Torsin.HA} and P{UASp-Torsin.E177Q.HA} constructs and insertions.

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Geyer, P. (2024.11.19). P{UASp-Torsin.HA} and P{UASp-Torsin.E177Q.HA} constructs and insertions.

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FBrf0260977

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Personal communication to FlyBase

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The following information accompanied stocks donated to the Bloomington Stock Center by Pam Geyer, University of Iowa.
P{UASp-Torsin.HA} is a pP{UASp} construct that expresses HA-tagged Torsin protein under UASp control.
P{UASp-Torsin.E177Q.HA} is an identical construct except the Torsin coding sequence has been mutated to introduce an E177Q substitution, which prevents ATP hydrolysis similar to P{UAS-Torsin.E177Q.mGFP} and P{UAS-Torsin.E177Q}.
P{UASp-Torsin.HA}3 and P{UASp-Torsin.E177Q.HA}3 are third chromosome insertions.
The constructs and insertions were generated by Vivian Budnik and colleagues.

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English

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