Abstract
Edible plant oils with a high n-3/n-6 polyunsaturated fatty acids (PUFAs) ratio exhibit numerous health benefits, potentially due to their ability to modulate cellular lipidomes metabolism within the organism. To test this hypothesis, lifespan studies in Drosophila were conducted to assess the impact of 7 representative plant oils with different n-3/n-6 PUFA ratios on health outcomes. Subsequently, multi-dimensional MS-based shotgun lipidomics was utilized for class-targeted lipid analysis of cellular lipidomes in fly bodies. The plant oils with high n-3/n-6 PUFAs ratio significantly extended the lifespan of Drosophila, enhancing overall health. Lipidomics analysis revealed that these oils substantially increased the composition of 18:3 free FA, reduced compositions of phospholipid species containing 18:2 FA in flies, and enhanced mitochondrial functions by elevating T18:2 cardiolipin composition. The study provides insights into the mechanism(s) underlying the positive health effects of plant oils with high n-3/n-6 PUFAs ratio.
