The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Georgios Pyrowolakis, University of Freiburg.
P{UAS-scFv(antiHA-X15F11).mEGFP} - UAS sequences regulate expression of the anti-HA 'frankenbody' scFv(antiHA-X15F11) tagged with mEGFP(A206K). Generated by removing the loxP-flanked lexAop sequences from P{lexAop-UAS-scFv(antiHA-X15F11).mEGFP} (FBtp0147825) using cre recombinase.
P{UAS-scFv(antiHA-X15F11).mEGFP}attP2 is an insertion on the third chromosome into attP2 (FBti0040535).
P{lexAop-scFv(antiHA-X15F11).mEGFP} - lexAop sequences regulate expression of the anti-HA 'frankenbody' scFv(antiHA-X15F11) tagged with mEGFP(A206K). Generated by removing the lox2272-flanked UAS sequences from P{lexAop-UAS-scFv(antiHA-X15F11).mEGFP} (FBtp0147825) using cre recombinase.
P{lexAop-scFv(antiHA-X15F11).mEGFP}attP2 is an insertion on the third chromosome into attP2 (FBti0040535).
P{UAS-deGradHA} - UAS sequences regulate expression of deGradHA for degrading HA-tagged proteins. Generated by removing the loxP-flanked lexAop sequences from P{lexAop-UAS-deGradHA} (FBtp0147823) using cre recombinase.
P{UAS-deGradHA}attP2 is an insertion on the third chromosome into attP2 (FBti0040535).
P{lexAop-deGradHA} - lexAop sequences regulate expression of deGradHA for degrading HA-tagged proteins. Generated by removing the lox2272-flanked UAS sequences from P{lexAop-UAS-deGradHA} (FBtp0147823) using cre recombinase.
P{lexAop-deGradHA}attP2 is an insertion on the third chromosome into attP2 (FBti0040535).
P{UAS-GrabHA.ext.mCh} - UAS sequences regulate expression of a fusion protein composed of an anti-HA 'frankenbody' scFv(anti-HA-scFvX15F11), a transmembrane domain ( Tag:M (mCd8a)) and mCherry. The anti-HA frankenbody binds HA-tagged extracellular proteins, while mCherry is located in the cytosol. Generated by removing the loxP-flanked lexAop sequences from P{lexAop-UAS-GrabHA.ext.mCh} (FBrf0260932) using cre recombinase.
P{UAS-GrabHA.ext.mCh}attP2 is an insertion on the third chromosome into attP2 (FBti0040535).
P{lexAop-GrabHA.ext.mCh} - lexAop sequences regulate expression of a fusion protein composed of an anti-HA 'frankenbody' scFv(anti-HA-scFvX15F11), a transmembrane domain ( Tag:M (mCd8a)) and mCherry. The anti-HA frankenbody binds HA-tagged extracellular proteins, while mCherry is located in the cytosol. Generated by removing the lox2272-flanked UAS sequences from P{lexAop-UAS-GrabHA.ext.mCh} (FBrf0260932) using cre recombinase.
P{lexAop-GrabHA.ext.mCh}attP2 is an insertion on the third chromosome into attP2 (FBti0040535).
P{UAS-GrabHA.int.mCh} - UAS sequences regulate expression of a fusion protein composed of mCherry, a transmembrane domain ( Tag:M (mCd8a)) and an anti-HA 'frankenbody' scFv(anti-HA-scFvX15F11). The anti-HA frankenbody binds HA-tagged intracellular proteins, while mCherry is extracellular. Generated by removing the loxP-flanked lexAop sequences from P{lexAop-UAS-GrabHA.int.mCh} (FBrf0260932) using cre recombinase.
P{UAS-GrabHA.int.mCh}attP2 is an insertion on the third chromosome into attP2 (FBti0040535).
P{lexAop-GrabHA.int.mCh} - lexAop sequences regulate expression of a fusion protein composed of mCherry, a transmembrane domain ( Tag:M (mCd8a)) and an anti-HA 'frankenbody' scFv(anti-HA-scFvX15F11). The anti-HA frankenbody binds HA-tagged intracellular proteins, while mCherry is extracellular. Generated by removing the lox2272-flanked UAS sequences from P{lexAop-UAS-GrabHA.int.mCh} (FBrf0260932) using cre recombinase.
P{lexAop-GrabHA.int.mCh}attP2 is an insertion on the third chromosome into attP2 (FBti0040535).
P{UAS-GrabHA.ECM.mCh} - UAS sequences regulate a fusion protein composed of anti-HA 'frankenbody' scFv(anti-HA-scFvX15F11) that localizes to the extracellular matrix of the basal lamina. The fusion protein contains the vkg coding sequence with an mCherry fluorescent tag and the anti-HA frankenbody inserted between the first and second exon. Generated by removing the loxP-flanked lexAop sequences from P{lexAop-UAS-GrabHA.ECM.mCh} (FBrf0260932) using cre recombinase.
P{UAS-GrabHA.ECM.mCh}attP2 is an insertion on the third chromosome into attP2 (FBti0040535).
P{lexAop-GrabHA.ECM.mCh} - lexAop sequences regulate a fusion protein composed of anti-HA 'frankenbody' scFv(anti-HA-scFvX15F11) that localizes to the extracellular matrix of the basal lamina. The fusion protein contains the vkg coding sequence with an mCherry fluorescent tag and the anti-HA frankenbody inserted between the first and second exon. Generated by removing the lox2272-flanked UAS sequences from P{lexAop-UAS-GrabHA.ECM.mCh} (FBrf0260932) using cre recombinase.
P{lexAop-GrabHA.ECM.mCh}attP2 is an insertion on the third chromosome into attP2 (FBti0040535).
(FlyBase curator comment: the descriptions and symbols for the GrabHA 'int' and 'ext' constructs have been updated from the original text to reflect the fact that they do not contain EGFP. date: 250627).