Abstract
With increasing concern on the problem of aging, search for food-derived anti-aging compounds has become a hot research area. This study investigated the anti-aging potential and underlying mechanisms of queen bee larvae enzymatic hydrolysates (QBLE) in Drosophila through measurement of the natural lifespan and the mRNA level of related signaling pathways and widely targeted metabolomics analysis based on UPLC-MS/MS. The results showed that QBLE could prolong the mean, median, and maximum lifespan of Drosophila. Particularly, supplementation of QBLE at 5 mg/mL increased the median and mean lifespan by 12.74 % and 15.04 %, respectively, indicating that QBLE is effective in prolonging lifespan of Drosophila. Moreover, QBLE supplementation significantly improved the climbing ability and gut integrity, as well as effectively reduced MDA accumulation and increased the levels of CAT, GSH, T-SOD, and T-AOC in aged Drosophila. The effect of QBLE to extend the lifespan was jointly influenced by the intrinsic stress protection system (Nrf2/Keap1), TOR pathway (TOR/S6K, PI3K/Akt/TOR), autophagy-related genes (Atg8a, Atg5), and longevity genes (MTH). Furthermore, differential metabolite analysis revealed that QBLE mitigates Drosophila aging by regulating arginine biosynthesis, glycerophosphlipid metabolism, pyrimidine metabolism, purine metabolism, and cysteine, glycine, and methionine metabolism. Overall, these findings suggest that QBLE has great potential to be applied in the health food and biomedicine fields as a novel protein-derived anti-aging agent.
