Trypanosoma brucei Tim50 have been shown to possesses phosphatase activity important for function (PMID:23212919). Human Tim50 has also been shown to have some phosphatase activity but it is not thought to be physiologically relevant (PMID:15044455). Other Tim50 orthologs have not been shown to possess phosphatase activity, lacking the canonical DxDxT active site motif associated with the prototypic Fcp1 phosophatase (PMID:12556522).
Alignment of the CTD family of putative protein serine/threonine phosphatases shows that the Tim50 family members lack a DxDxT motif and are therefore members of the PSEUDOPHOSPHATASES gene group:
FBgn0035124	ttm2
FBgn0032971	ttm3
FBgn0250874	ttm50
The candidates that do contain this motif and are therefore are placed in the CTD FAMILY PROTEIN SERINE/THREONINE PHOSPHATASES gene group:
FBgn0035426	CG12078
FBgn0033322	CG8584
FBgn0029067	Ctdnep1
FBgn0035026	Fcp1
FBgn0027526	Ublcp1
FBgn0036556	hzg