The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Junjie Luo and Mark Schnitzer, Stanford University.
M{SuRe.AD1(Bxb1)1} is a SuRe R1 Recombinator construct carrying an Adaptor1 (AD1) with a Bxb1 attP site for Bxb1-mediated recombination and a gRNA that targets a sequence (ACCGTCGACGATGTAGGTCANGG) present in most Janelia-based vectors upstream of the transgene. This AD1 carries 2xr4-mCherry, the Bxb1 attP site and a Su(Hw) insulator.
M{SuRe.AD1(Bxb1)1}ZH-2A is a viable insertion on the X chromosome into ZH-2A (FBti0076477).
PBac{SuRe.AD2(Bxb1)1} is a SuRe R2 Recombinator construct carrying an Adaptor2 (AD2) for with a Bxb1 attB site for Bxb1-mediated recombination and a gRNA that targets a sequence (CCGCAGTCCGATCATCGGATNGG) in the mini-w+ marker downstream of the target transgene in most Janelia-based vectors. This AD2 carries the Bxb1 attB site and a 2xr4-CFP marker.
PBac{SuRe.AD2(Bxb1)1}VK00027 is a viable insertion on the third chromosome into VK00027 (FBti0076447).
PBac{SuRe.AD2(Bxb1)1}VK00033 is a viable insertion on the third chromosome into VK00033 (FBti0076453).
M{SuRe.AD2(Bxb1)4}is a SuRe R2 Recombinator construct carrying an Adaptor2 (AD2) with a Bxb1 attB site for Bxb1-mediated recombination. This AD2 carries the marker 2xr4-CFP, a gRNA that targets the sequence (CACCGCGCCCAGCGGTTTCGNGG), and a homology region located downstream of the attB site. It differs from M{SuRe.AD2(Bxb1)3}ZH-2A in that the insertion is marked with 3xP3-DsRed rather than 2xr4-DsRed for use with target insertions that carry 3xP3-RFP.
M{SuRe.AD2(Bxb1)4}ZH-2A is a viable insertion on the X chromosome into ZH-2A (FBti0076477). It differs from M{SuRe.AD2(Bxb1)3}ZH-2A in that the insertion is marked with 3xP3-DsRed rather than 2xr4-DsRed. Use M{SuRe.AD2(Bxb1)4} to insert AD2 into compatible insertions carrying a 3xP3-RFP marker.