FB2026_02 , released June 18, 2026
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Luo, J., Schnitzer, M. (2026.2.17). SuRe system constructs and insertions from Junjie Luo and Mark Schnitzer. 
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FBrf0264662
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The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Junjie Luo and Mark Schnitzer, Stanford University.
1. M{AD1(Bxb1),vas-int.Dm} carries phiC31 integrase under the control of the vas promoter (derived from M{vas-int.Dm}ZH-2A with the 3xP3-RFP and 3xP3-GFP markers removed by Cre recombinase). Also carries a 2xr4-mCherry marked AD1(Bxb1) attP adaptor for use in the SuRe system. 
M{AD1(Bxb1),vas-int.Dm}ZH-2A is a homozygous and hemizygous viable and fertile insertion on the X chromosome into ZH-2A (FBti0076477).
2. M{AD1(Bxb1),vas-Bxb1.nanos3'UTR} carries Bxb1 recombinase expressed in the germ line under the control of vasa and nanos regulatory sequences. The insertion is derived from M{vas-Bxb1.nos3'UTR} with the 3xP3-RFP marker removed. Also carries a 2xr4-mCherry marked AD1(Bxb1) attP adaptor for use in the SuRe system.
M{AD1(Bxb1),vas-Bxb1.nanos3'UTR}ZH-2A is a homozygous and hemizygous viable and fertile insertion on the X chromosome into ZH-2A (FBti0076477).
3. M{AD1(Bxb1),vas-NLS-TP901-1.nanos3'UTR} carries a nuclear-localized Drosophila codon-optimized TP901-1 integrase under the control of vasa and nanos regulatory sequences. Derived from M{vas-NLS-TP901-1.nanos3'UTR.RFP-.GFP-}ZH-2A.  Also carries a 2xr4-mCherry marked AD1(Bxb1) attP adaptor for use in the SuRe system.
M{AD1(Bxb1),vas-NLS-TP901-1.nanos3'UTR}ZH-2A is a homozygous and hemizygous viable and fertile insertion on the X chromosome into ZH-2A (FBti0076477).
4. M{AD1(Bxb1),Act5C-Cas9.P} carries Cas9 endonuclease expressed ubiquitously under the control of Act5C. Derived from M{Act5C-Cas9.P.RFP-}ZH-2A. Also carries a 2xr4-mCherry marked AD1(Bxb1) attP adaptor for use in the SuRe system.
M{AD1(Bxb1),Act5C-Cas9.P}ZH-2A is a homozygous and hemizygous viable and fertile insertion on the X chromosome into ZH-2A (FBti0076477).
5. M{AD1(Bxb1),nanos-Cas9.P} carries Cas9 endonuclease expressed during oogenesis under the control of nanos regulatory sequences (derived from M{nanos-Cas9.P}ZH-2A). Also carries a 2xr4-mCherry marked AD1(Bxb1) attP adaptor for use in the SuRe system.
M{AD1(Bxb1),nanos-Cas9.P}ZH-2A is a homozygous and hemizygous viable and fertile insertion on the X chromosome into ZH-2A (FBti0076477).
6. M{vas-Bxb1.nanos3'UTR,AD2(Bxb1)} carries Bxb1 recombinase expressed in the germ line under the control of vasa and nanos regulatory sequences. The insertion is derived from M{vas-Bxb1.nos3'UTR} by removal of the 3xP3-RFP marker. Also carries a 2xr4-CFP marked AD2(Bxb1) attB adaptor for use in the SuRe system. 
M{vas-Bxb1.nanos3'UTR,AD2(Bxb1)}ZH-2A is a homozygous and hemizygous viable and fertile insertion on the X chromosome into ZH-2A (FBti0076477).
7. M{vas-Bxb1.nanos3'UTR,Act5C-Cas9.P.RFP-} carries Bxb1 recombinase expressed in the germ line under the control of vasa and nanos regulatory sequences and Cas9 endonuclease expressed ubiquitously under the control of Act5C. Derived from M{vas-Bxb1.nanos3'UTR.RFP-}ZH-2A and M{Act5C-Cas9.P.RFP-}ZH-2A.
M{vas-Bxb1.nanos3'UTR,Act5C-Cas9.P.RFP-}ZH-2A is a homozygous and hemizygous viable and fertile insertion on the X chromosome into ZH-2A (FBti0076477).
8. M{vas-Bxb1.nanos3'UTR,nanos-Cas9.P} carries Bxb1 recombinase expressed during oogenesis under the control of vasa and nanos regulatory sequences and Cas9 endonuclease expressed during oogenesis under the control of nanos regulatory sequences. Derived from M{vas-Bxb1.nanos3'UTR.RFP-}ZH-2A and M{nanos-Cas9.P}ZH-2A.
M{vas-Bxb1.nanos3'UTR,nanos-Cas9.P}ZH-2A is a homozygous and hemizygous viable and fertile insertion on the X chromosome into ZH-2A (FBti0076477).
9. M{vas-Bxb1.nanos3'UTR,vas-int.Dm} carries Bxb1 recombinase expressed during oogenesis under the control of vasa and nanos regulatory sequences and phiC31 integrase expressed during oogenesis under the control of vasa regulatory sequences. Derived from M{vas-Bxb1.nanos3'UTR.RFP-}ZH-2A and M{vas-int.Dm}ZH-2A.
M{vas-Bxb1.nanos3'UTR,vas-int.Dm}ZH-2A is a homozygous and hemizygous viable and fertile insertion on the X chromosome into ZH-2A (FBti0076477).
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