FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Recombinant Construct: M{vas-Bxb1.nanos3'UTR,Act5C-Cas9.P.RFP-}
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General Information
Symbol
M{vas-Bxb1.nanos3'UTR,Act5C-Cas9.P.RFP-}
FlyBase ID
FBtp0174619
Feature type
transgenic_transposable_element
Component Allele(s)
Bxb1\intvas.nanos3'UTR
Spyo\Cas9Act5C.cPa
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Bxb1\intvas.nanos3'UTR
Transgenic product class
Component
Use(s)
Regulatory region(s)
vas
Encoded product / tool
Bxb1:int
Description

vas promoter sequences drive expression of a cDNA encoding the Bxb1:int integrase, followed by the nos 3'UTR.

(Voutev and Mann, 2017)
Component allele Spyo\Cas9Act5C.cPa
Transgenic product class
Component
Use(s)
Regulatory region(s)
Act5C
Encoded product / tool
Cas9
Description

A 4.4 kb fragment of the upstream regulatory region of the Act5C gene drives expression of human codon optimized Cas9 endonuclease (cloned from Addgene plasmid 41815).

(Port and Bullock, 2014.1.10)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
607397
y1 M{vas-Bxb1.nanos3'UTR,Act5C-Cas9.P.RFP-}ZH-2A w1118
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
M{vas-Bxb1.nanos3'UTR,Act5C-Cas9.P.RFP-}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)