FlyBase Allele Report: Spyo\Cas9[Act5C.cPa]

A 4.4 kb fragment of the upstream regulatory region of the Act5C gene drives expression of human codon optimized Cas9 endonuclease (cloned from Addgene plasmid 41815).

(Port and Bullock, 2014.1.10)

Allele components

Component

Use(s)

Regulatory region(s)

Act5C

Encoded product / tool

Cas9

RNA-guided nuclease

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis | larval stage, with Insep^TKO.GS01105

(Kakade et al., 2025)

chemical sensitive | adult stage, with Ugt35B1^TKO.GS03380

(Pfannenstiel et al., 2026)

decreased size | larval stage, with Insep^TKO.GS01105

(Kakade et al., 2025)

flightless, with Flacc^TKO.GS00449

(Guo et al., 2018)

increased mortality, with Cpr49Ah^UAS.gRNA, Scer\GAL4^phtm.PO

(Liu et al., 2024)

increased mortality, with Scer\GAL4^phtm.PO, pdm3^UAS.gRNA

(Liu et al., 2024)

increased mortality, with Ugt35B1^TKO.GS03380

(Pfannenstiel et al., 2026)

lethal, with LpR1^{U6:3.2xgRNA.long}

(Yin et al., 2021)

lethal | female, with Flacc^TKO.GS00449

(Guo et al., 2018)

lethal - all die before end of second instar larval stage, with Insep^TKO.GS01105

(Kakade et al., 2025)

lethal - all die during second instar larval stage, with RNaseZ^U6.3xsgRNA

(Migunova et al., 2023)

partially lethal, with sloth1^sgRNA.JAB203

(Bosch et al., 2022)

partially lethal, with sloth2^sgRNA.JAB203

(Bosch et al., 2022)

partially lethal - majority die, with Cpr49Ah^UAS.gRNA, Scer\GAL4^phtm.PO

(Liu et al., 2024)

partially lethal - majority die, with LpR1^{U6:3.2xgRNA.short}

(Yin et al., 2021)

partially lethal - majority die, with Scer\GAL4^phtm.PO, pdm3^UAS.gRNA

(Liu et al., 2024)

segment polarity phenotype, with wg^{gRNA.U6:1-U6:2-U6:3.P1-3}

(Port et al., 2014)

some die during larval stage, with Cpr49Ah^UAS.gRNA, Scer\GAL4^phtm.PO

(Liu et al., 2024)

some die during larval stage, with Scer\GAL4^phtm.PO, pdm3^UAS.gRNA

(Liu et al., 2024)

some die during P-stage, with Cpr49Ah^UAS.gRNA, Scer\GAL4^phtm.PO

(Liu et al., 2024)

some die during P-stage, with Scer\GAL4^phtm.PO, pdm3^UAS.gRNA

(Liu et al., 2024)

visible | adult stage, with Flacc^TKO.GS00449

(Guo et al., 2018)

visible | adult stage, with sloth1^sgRNA.JAB203

(Bosch et al., 2022)

visible | adult stage, with sloth2^sgRNA.JAB203

(Bosch et al., 2022)

Phenotype Manifest In

cytoplasmic vesicle | increased number | larval stage, with Insep^TKO.GS01105

(Kakade et al., 2025)

endoplasmic reticulum | larval stage, with Insep^TKO.GS01105

(Kakade et al., 2025)

larval brain, with Insep^TKO.GS01105

(Kakade et al., 2025)

larval neuroblast, with Insep^TKO.GS01105

(Kakade et al., 2025)

scutellar bristle, with sloth1^sgRNA.JAB203

(Bosch et al., 2022)

scutellar bristle, with sloth2^sgRNA.JAB203

(Bosch et al., 2022)

wing, with Flacc^TKO.GS00449

(Guo et al., 2018)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Insep^TKO.GS01105, Spyo\Cas9^Act5C.cPa has decreased size | larval stage phenotype, suppressible by Hsap\IER3IP1^{UAS.cKa.Tag:HA}/Scer\GAL4^Tub.PU

(Kakade et al., 2025)

NOT suppressed by

Statement

Reference

Insep^TKO.GS01105, Spyo\Cas9^Act5C.cPa has decreased size | larval stage phenotype, non-suppressible by Rab11^{Q70L.UASp.YFP}

(Kakade et al., 2025)

Insep^TKO.GS01105, Spyo\Cas9^Act5C.cPa has lethal - all die before end of second instar larval stage phenotype, non-suppressible by Rab11^{Q70L.UASp.YFP}

(Kakade et al., 2025)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Insep^TKO.GS01105, Spyo\Cas9^Act5C.cPa has larval brain phenotype, non-suppressible by Rab11^{Q70L.UASp.YFP}

(Kakade et al., 2025)

Insep^TKO.GS01105, Spyo\Cas9^Act5C.cPa has larval brain phenotype, non-suppressible by Hsap\IER3IP1^{UAS.cKa.Tag:HA}/Scer\GAL4^Tub.PU

(Kakade et al., 2025)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (56)

Bloomington

54590

y¹ M{Act5C-Cas9.P}ZH-2A w^*

66489

y¹ M{Act5C-Cas9.P}ZH-2A w^*; P{QF2.G4HACK}28E4

66493

y¹ M{Act5C-Cas9.P}ZH-2A w^*; P{QF2.G4HACK}57C1

66494

y¹ M{Act5C-Cas9.P}ZH-2A w^*; P{QF2.G4HACK}64B5

66495

y¹ M{Act5C-Cas9.P}ZH-2A w^*; P{QF2.G4HACK}65E3

66496

y¹ M{Act5C-Cas9.P}ZH-2A w^*; P{QF2.G4HACK}70F4

66497

y¹ M{Act5C-Cas9.P}ZH-2A w^*; P{QF2.G4HACK}79A2

66498

y¹ M{Act5C-Cas9.P}ZH-2A w^*; P{QF2.G4HACK}84C8

66502

y¹ M{Act5C-Cas9.P}ZH-2A w^*; P{QF2.G4HACK}96D1

66499

y¹ M{Act5C-Cas9.P}ZH-2A w^*; P{QF2.G4HACK}86E11

66504

y¹ M{Act5C-Cas9.P}ZH-2A w^*; P{QF2.G4HACK}attP2

66487

y¹ M{Act5C-Cas9.P}ZH-2A w^*; P{QF2.G4HACK}23F3/CyO

More stocks available...

Notes on Origin

Discoverer

Comments

Zzzz\Cas9^Act5C.cPa can induce mutations in somatic and germ-line cells with high frequency.

(Port et al., 2014)

Generated by Fillip Port and Simon Bullock, Medical Research Council, Cambridge.

(Port and Bullock, 2014.1.10)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Spyo\Cas9^Act5C.cPa

Zzzz\Cas9^Act5C.cPa

Name Synonyms

Actin 5C construct a of Port

Secondary FlyBase IDs

FBal0320670
FBal0294140

References (37)

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