FlyBase Allele Report: Dmel\Insep[TKO.GS01105]

General Information

Symbol

Dmel\Insep^TKO.GS01105

Species

D. melanogaster

Name

FlyBase ID

FBal0336496

Feature type

allele

Associated gene

Dmel\Insep

Associated Insertion(s)

Carried in Construct

P{TKO.GS01105}

Key Links

Transgenic product class

sgRNA

(Transgenic RNAi Project members, 2017-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

sgRNA

(Transgenic RNAi Project members, 2017-)

Mutagen

in vitro construct

(Transgenic RNAi Project members, 2017-)

Progenitor genotype

Carried in construct

P{TKO.GS01105}

Cytology

Description

CRISPR construct designed to result in an indel (small insertion or deletion mutation) in a target gene when combined with a source of Cas9. Constitutively expresses an sgRNA that targets the coding sequence of CG32069.

(Transgenic RNAi Project members, 2017-)

Allele components

Component

Use(s)

Encoded product / tool

sgRNA-GS01105

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis | larval stage, with Spyo\Cas9^Act5C.cPa

(Kakade et al., 2025)

decreased size | larval stage, with Spyo\Cas9^Act5C.cPa

(Kakade et al., 2025)

lethal - all die before end of second instar larval stage, with Spyo\Cas9^Act5C.cPa

(Kakade et al., 2025)

Phenotype Manifest In

cytoplasmic vesicle | increased number | larval stage, with Spyo\Cas9^Act5C.cPa

(Kakade et al., 2025)

endoplasmic reticulum | larval stage, with Spyo\Cas9^Act5C.cPa

(Kakade et al., 2025)

larval brain, with Spyo\Cas9^Act5C.cPa

(Kakade et al., 2025)

larval neuroblast, with Spyo\Cas9^Act5C.cPa

(Kakade et al., 2025)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Insep^TKO.GS01105, Spyo\Cas9^Act5C.cPa has decreased size | larval stage phenotype, suppressible by Hsap\IER3IP1^{UAS.cKa.Tag:HA}/Scer\GAL4^Tub.PU

(Kakade et al., 2025)

NOT suppressed by

Statement

Reference

Insep^TKO.GS01105, Spyo\Cas9^Act5C.cPa has decreased size | larval stage phenotype, non-suppressible by Rab11^{Q70L.UASp.YFP}

(Kakade et al., 2025)

Insep^TKO.GS01105, Spyo\Cas9^Act5C.cPa has lethal - all die before end of second instar larval stage phenotype, non-suppressible by Rab11^{Q70L.UASp.YFP}

(Kakade et al., 2025)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Insep^TKO.GS01105, Spyo\Cas9^Act5C.cPa has larval brain phenotype, non-suppressible by Rab11^{Q70L.UASp.YFP}

(Kakade et al., 2025)

Insep^TKO.GS01105, Spyo\Cas9^Act5C.cPa has larval brain phenotype, non-suppressible by Hsap\IER3IP1^{UAS.cKa.Tag:HA}/Scer\GAL4^Tub.PU

(Kakade et al., 2025)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

77269

y¹ sc^* v¹ sev²¹; P{TKO.GS01105}attP40

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG32069^TKO.GS01105

Insep^TKO.GS01105

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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