The following information accompanied stocks donated to the Bloomington Stock Center by Fillip Port and Simon Bullock, Medical Research Council, Cambridge.
In M{Act5C-Cas9.P}, a human codon optimised Cas9 sequence from the Church lab (Addgene plasmid 41815) was cloned behind a 4.4 kb fragment of the upstream regulatory region of the Act5C gene. It supports strong ubiquitous expression of Cas9 protein.       
M{Act5C-Cas9.P}ZH-2A is an insertion in the M{3xP3-RFP.attP}ZH-2A target site at 2A3,  X:1345925  (R5).
In M{nos-Cas9.P}, a human codon optimised Cas9 sequence from the Church lab (Addgene plasmid 41815) was cloned into a plasmid containing the nanos promoter and 3' UTR to give germline specific expression. Its construction was similar to that of M{nos-int.B} (FBtp0023081).
M{nos-Cas9.P}ZH-2A is an insertion in the M{3xP3-RFP.attP}ZH-2A target site at 2A3,  X:1345925  (R5).
P{UAS-Cas9.P} has a Drosophila codon-optimised Cas9 sequence fused to the nuclear localization signal sequence of Ude (FBgn0039226) cloned into P{10XUAS-IVS-Syn21-GFP-p10} (pJFRC81; FBtp0073107) described in Pfeiffer et al. (FBrf0218136), which has been shown to drive strong expression in both somatic and germ cells.
P{UAS-Cas9.P}attP2 is an insertion in the P{CaryP}attP2 target site at 68A4,  3L:11063638  (R5).
P{UAS-Cas9.P}attP40 is an insertion in the P{CaryP}attP40 target site at 25C6,  2L:5108448  (R5).
P{UAS-Cas9.C} has a Drosophila codon-optimised Cas9 sequence with N- and C-terminal nuclear localization signal sequences cloned into the KpnI and XbaI restriction sites of P{10XUAS-IVS-GFP-p10} (pJFRC28; FBtp0073105) described in Pfeiffer et al. (FBrf0218136).
P{UAS-Cas9.C}attP2 is an insertion in the P{CaryP}attP2 target site at 68A4,  3L:11063638  (R5).
P{UAS-Cas9.D10A} is similar to P{UAS-Cas9.C} with a site-directed mutation changing aspartate 10 to alanine.  This converts Cas9 into a single-strand nickase.
P{UAS-Cas9.D10A}attP2 is an insertion in the P{CaryP}attP2 target site at 68A4,  3L:11063638  (R5).
P{UAS-Cas9.C}and P{UAS-Cas9.D10A} constructs and insertions were generated by Hui-Min Chen in the laboratory of Tzumin Lee, Janelia Farm Research Campus.