FlyBase Recombinant Construct Report: M{nanos-Cas9.P}

General Information

Symbol

M{nanos-Cas9.P}

FlyBase ID

FBtp0092338

Feature type

transgenic_transposable_element

Component Allele(s)

Spyo\Cas9^nanos.cPa

Also Known As

nos-cas9

Size

Expression Data

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component allele Spyo\Cas9^nanos.cPa

Transgenic product class

Component

Use(s)

Regulatory region(s)

nanos

Encoded product / tool

Cas9

RNA-guided nuclease

Description

A human codon-optimized Cas9 endonuclease (cloned from Addgene plasmid 41815) is expressed under the control of nos promoter and 3' UTR sequences to give germline specific expression.

(Port and Bullock, 2014.1.10)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Spyo\Cas9^nanos.cPa

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Zzzz\Cas9 is derived from Streptococcus pyogenes.

(Port and Bullock, 2014.1.10)

Stocks (2)

Stocks containing insertion(s) of this recombinant construct

Bloomington

54591

y¹ M{nanos-Cas9.P}ZH-2A w^*

80093

y¹ M{nanos-Cas9.P}ZH-2A w^*; TI{y^wing2+}CG13390^ΔCDS/CyO

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

M{nanos-Cas9.P}

(FlyBase, 1996-)

M{nos-Cas9.P}

(Port and Bullock, 2014.1.10, FlyBase, 1992-)

nanos-Cas9

(Mohana et al., 2023)

nos-Cas9

(Chen et al., 2025, Akpoghiran et al., 2024, Han et al., 2023, Karuparti et al., 2023, Allen et al., 2021, Douthit et al., 2021, Koreman et al., 2021, Tamada et al., 2021, Hu et al., 2020, Maselko et al., 2020, Trivedi et al., 2020, Meltzer et al., 2019)

nos-SpCas9

(López Del Amo et al., 2020)

nos-cas9

(Madan et al., 2023, Port et al., 2015, Port et al., 2014)

nosCas9

(Warsinger-Pepe et al., 2023)

nosP-Cas9

(Chen et al., 2020)

Secondary FlyBase IDs

References (25)

Report Sections

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