FlyBase Recombinant Construct Report: P{UAS-Cas9.C}

A Drosophila codon-optimized Cas9 endonuclease with N- and C-terminal nuclear localization signal sequences is expressed under the control of UAS regulatory sequences (ORF cloned into the KpnI and XbaI restriction sites of P{10XUAS-IVS-GFP-p10}, FBtp0073105).

(Port and Bullock, 2014.1.10)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Spyo\Cas9^{UAS.Tag:NLS(Unk)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Zzzz\Cas9 is derived from Streptococcus pyogenes.

(Port and Bullock, 2014.1.10)

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

54595

w¹¹¹⁸; P{UAS-Cas9.C}attP2

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CFD5

(Port et al., 2014)

P{UAS-Cas9.C}

(Hürkey et al., 2023, Port and Bullock, 2014.1.10, FlyBase, 1992-)

UAS-CAS9.c

(Ryvkin et al., 2021)

UAS-Cas9

(González-Gutiérrez et al., 2025, Bazzi et al., 2023, González Morales et al., 2023, Liu et al., 2023, Mayseless et al., 2023, Xu et al., 2022, Christesen et al., 2021, Yin et al., 2021, Hu and Wolfner, 2019, Hu et al., 2019, Kudumala et al., 2017)

UAS-Cas9.C

(Bornstein et al., 2021, Meltzer et al., 2019, Huynh et al., 2018)

UAS-cas9

(Jiang et al., 2025, Port et al., 2014)

Secondary FlyBase IDs

References (21)

Report Sections

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