FB2026_02 , released June 18, 2026
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Li, X., Li, Y. (2026.5.12). GRAB sensor tools and insertions from Xuelin Li and Yulong Li. 
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FBrf0265388
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The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Xuelin Li and Yulong Li, Peking University.
P{10XUAS-GRAB(rACh1h)} expresses the acetylcholine sensor, rACh1h, under UAS control.
P{10XUAS-GRAB(rACh1h)}attP40 is a viable insertion on the second chromosome into attP40 (FBti0114379).
The GRAB-ACh4.0 series sensors (ACh4l, ACh4m, and ACh4h) were all developed based on the human M3R (CHRM3) receptor, the same as GRAB-ACh3.0. The circularly permuted fluorescent protein in all three sensors is cpEGFP. The three sensors  (ACh4l, ACh4m, and ACh4h) have low, medium and high affinity to acetylcholine respectively.
PBac{10XUAS-GRAB(ACh4m)} expresses the acetylcholine sensor, ACh4m, under UAS control.
PBac{10XUAS-GRAB(ACh4m)}VK00005 is a viable insertion on the third chromosome into VK00005 (FBti0076428).
P{10XUAS-GRAB(ACh4l)} expresses the acetylcholine sensor, ACh4l, under UAS control.
P{10XUAS-GRAB(ACh4l)}attP40 is a viable insertion on the second chromosome into attP40 (FBti0114379).
P{10XlexAop-GRAB(ACh4h)} expresses the acetylcholine sensor, ACh4h, under lexAop control.
P{10XlexAop-GRAB(ACh4h)}attP40 is an insertion on the second chromosome into attP40 (FBti0114379).
PBac{10XUAS-GRAB(DA3m)} expresses the dopamine sensor, DA3m, under UAS control.
PBac{10XUAS-GRAB(DA3m)}VK00005 is a viable insertion on the third chromosome into VK00005 (FBti0076428).
P{10XlexAop-GRAB(DA3m)} expresses the dopamine sensor, DA3m, under lexAop control.
P{10XlexAop-GRAB(DA3m)}attP40 is a viable insertion on the second chromosome into attP40 (FBti0114379).
P{10XUAS-GRAB(DA3h)} expresses the dopamine sensor, DA3h, under UAS control.
P{10XUAS-GRAB(DA3h)}attP40 is a viable insertion on the second chromosome into attP40 (FBti0114379).
P{10XlexAop-GRAB(DA3h)} expresses the dopamine sensor, DA3h, under lexAop control.
P{10XlexAop-GRAB(DA3h)}attP40 is a viable insertion on the second chromosome into attP40 (FBti0114379).
P{10XUAS-GRAB(rDA3m)} expresses the dopamine sensor, rDA3m, under UAS control.
P{10XUAS-GRAB(rDA3m)}attP40 is a viable insertion on the second chromosome into attP40 (FBti0114379).
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