FlyBase Insertion Report: Dmel\P{PZ}svp[07842]

General Information

Symbol

Dmel\P{PZ}svp⁰⁷⁸⁴²

Species

D. melanogaster

Name

FlyBase ID

FBti0003610

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)07842

Affected gene(s)

Ecol\lacZ, svp

Viability / fertility

Associated allele(s)

Ecol\lacZ^svp-07842, svp⁰⁷⁸⁴²

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 87B5 )

Sequence Location

3R:12,281,086..12,281,086 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:12,281,086..12,281,086 [+]

Orientation

Cytological location (computed by FlyBase)

87B5 (inferred by FlyBase from sequence location)

Cytological location (reported)

87B4-87B6 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

G00472

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^svp-07842

Phenotypic Class Phenotype Manifest in Detailed Description

svp⁰⁷⁸⁴²

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval fat body

(Meister and Braun, 1995.10)

Malpighian tubule

(Meister and Braun, 1995.10)

pupal stage P5(ii)

proneural cluster of antennal disc | restricted | subset

(Sen et al., 2003)

external sensory organ precursor cell IIb

(Sen et al., 2003)

sense organ glial cell | faint | subset of coeloconic sensillum

(Sen et al., 2003)

pupal stage P6

external sensory organ precursor cell IIIb

(Sen et al., 2003)

pupal stage P7

thecogen cell | subset

(Sen et al., 2003)

eo neuron | subset

(Sen et al., 2003)

adult stage

third segment of antenna

(Vosshall, 1998.8.10)

adult optic lobe

(Vosshall, 1998.8.10)

photoreceptor cell R1

(Wernet et al., 2003)

photoreceptor cell R6

(Wernet et al., 2003)

adult fat body

(Vosshall, 1998.8.10)

photoreceptor cell R4

(Wernet et al., 2003)

photoreceptor cell R3

(Wernet et al., 2003)

Additional Information

Statement

Reference

Strong expression is observed in photoreceptor cells R3 and R4, and weak expression in photoreceptor cells R1 and R6.

(Wernet et al., 2003)

Expression of EcollacZ^svp-07842 in the eo glial cell is transient and is rapidly lost as the cell moves away to associate with the fasciculating sensory neuron. Expression of EcollacZ^svp-07842 in the thecogen (socket) cell is no longer detectable by 36hrs APF.

(Sen et al., 2003)

In third instar larva, high levels of expression in fat body, low levels in Malpighian tubules.

(Meister and Braun, 1995.10)

Marker for

photoreceptor cell R1

(Wernet et al., 2003)

photoreceptor cell R3

(Wernet et al., 2003)

photoreceptor cell R4

(Wernet et al., 2003)

photoreceptor cell R6

(Wernet et al., 2003)

Reflects expression of

svp

(Sen et al., 2003, Wernet et al., 2003)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)07842

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11725

P{PZ}svp⁰⁷⁸⁴² ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

P1725

(Meister and Braun, 1995.10)

P[lacW]svp⁰⁷⁸⁴²

(Brown and Freeman, 2003)

P{PZ}l(3)07842⁰⁷⁸⁴²

(FlyBase, 1992-)

P{PZ}svp⁰⁷⁸⁴²

(Begemann et al., 1995, FlyBase, 1992-)

P{lacZ}svp⁰⁷⁴⁸²

(Fanto et al., 1998, FlyBase, 1992-)

P{ry^+t7.2=PZ}svp⁰⁷⁸⁴²

(FlyBase, 1992-)

svp-PZ

(Wernet et al., 2003)

svp-lacZ

(Takacs et al., 2008, Montrasio et al., 2007)

svp⁰⁷⁴⁸²-PZ

(Benchabane et al., 2011)

svp⁰⁷⁴⁸²-lacZ

(Takacs et al., 2008)

svp^P1725

(Sen et al., 2003)

Name Synonyms

Secondary FlyBase IDs

FBti0000742
FBti0012160

References (31)

Report Sections

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