FlyBase Insertion Report: Dmel\P{PZ}grn[05930]

General Information

Symbol

Dmel\P{PZ}grn⁰⁵⁹³⁰

Species

D. melanogaster

Name

FlyBase ID

FBti0004156

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)05930

Affected gene(s)

Ecol\lacZ, grn

Viability / fertility

Associated allele(s)

Ecol\lacZ^grn-05930, grn⁰⁵⁹³⁰

Stock availability

None publicly available

Genomic Location

Chromosomal Location

3R ( 84F1 )

Sequence Location

3R:8,181,477..8,181,477 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:8,181,477..8,181,477 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

84F1 (inferred by FlyBase from sequence location)

Cytological location (reported)

84F1-84F2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ034067

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Garces and Thor, 2006, BDGP Project Members, 1994-1999)

grn

(Garces and Thor, 2006, Lin et al., 1995)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^grn-05930

Phenotypic Class Phenotype Manifest in Detailed Description

grn⁰⁵⁹³⁰

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 12 -- 14

larval DA1 motor neuron

(Garces and Thor, 2006)

larval RP2 motor neuron

(Garces and Thor, 2006)

larval DO1 motor neuron

(Garces and Thor, 2006)

larval DO2 motor neuron

(Garces and Thor, 2006)

larval DA2 motor neuron

(Garces and Thor, 2006)

larval DA3 motor neuron

(Garces and Thor, 2006)

larval LL1 motor neuron

(Garces and Thor, 2006)

Additional Information

Statement

Reference

In the ventral nerve cord, grn reporter expression begins at early embryonic stage 12 in a diverse set of interneurons and motorneurons that extend axons along the major axon tracts. With the exception of one doral glial cell, expression is restricted to neurons. Based on overlap with neural markers, expression was identified in the intersegmental nerve motoneurons aCC, RP2 and the U1-5 neurons.

(Garces and Thor, 2006)

Marker for

Reflects expression of

grn

(Garces and Thor, 2006)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)05930

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

Location 3R:4007199-4007200 confirmed by FlyBase alignment of dbGSS accession AQ034067 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

P{PZ}Gata-c⁰⁵⁹³⁰

(Lin et al., 1995, FlyBase, 1992-)

P{PZ}grn⁰⁵⁹³⁰

(FlyBase, 2005, FlyBase, 1992-)

grn^lacZ

(Garces and Thor, 2006)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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