FB2026_02 , released June 18, 2026
Insertion: Dmel\P{lacW}14-3-3εj2B10
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General Information
Symbol
Dmel\P{lacW}14-3-3εj2B10
Species
D. melanogaster
Name
FlyBase ID
FBti0004920
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{lacW}
LINE ID

l(3)j2B10

Affected gene(s)
14-3-3ε, Ecol\lacZ
Viability / fertility
viable
Associated allele(s)
14-3-3εj2B10, Ecol\lacZ14-3-3ε-j2B10
Stock availability
2 publicly available
Genomic Location
Chromosomal Location
3R ( 90F10 )
Sequence Location
3R:18,244,702..18,244,709 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{lacW}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:18,244,702..18,244,709 [+]
(Chang and Rubin, 1997)
Orientation
Cytological location (computed by FlyBase)
90F10 (inferred by FlyBase from sequence location)
Cytological location (reported)
90F6-90F7 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location

Genomic location previously reported as 3R:14070424..14070424 .

(FlyBase, 2005)
Sequence Data
Flanking sequence
AQ026302
(FlyBase, 1992-)
Inserted Element
Construct
P{lacW}
Size (Kb)

10.691

Component Allele(s)
w+mC
Molecular map
Affected Gene(s)
Insertion may affect gene
14-3-3ε
(Chang and Rubin, 1997)
Ecol\lacZ
(BDGP Project Members, 1994-1999)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage
embryonic/larval midgut | restricted
(Meister and Braun, 1995.10)
embryonic/larval salivary gland
(Meister and Braun, 1995.10)
gastric caecum
(Meister and Braun, 1995.10)
third instar larval stage L2
Malpighian tubule | restricted
(Meister and Braun, 1995.10)
Additional Information
Statement
Reference

In wandering third instar larva, moderate expression in gastric caecum; lower levels in midgut, salivary glands and Malpighian tubules.

(Meister and Braun, 1995.10)
 
Marker for
Reflects expression of
14-3-3ε
(Meister and Braun, 1995.10)
Reporter construct used in assay
Data on Genetic Line
Line ID

l(3)j2B10

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 3R:14070423-14070424 determined by FlyBase alignment of dbGSS accession AQ026302 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
P2142
(Meister and Braun, 1995.10)
P{lacW}14-3-3εj2B10
(FlyBase, 2005, Chang and Rubin, 1997, FlyBase, 1992-)
P{lacW}14-3-3εj2B10</up>
(Chang and Rubin, 1997)
P{lacW}l(3)j2B10j2B10
(FlyBase, 1992-)
P{w+mC=lacW}l(3)j2B10j2B10
(FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
  • FBti0001168
References (22)