FB2026_03 , released September 17, 2026
Insertion: Dmel\P{PZ}vnrF264
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General Information
Symbol
Dmel\P{PZ}vnrF264
Species
D. melanogaster
Name
FlyBase ID
FBti0005059
Feature type
transposable_element_insertion_site
Also Known As
vn-lacZ
Description
Inserted Element
P{PZ}
LINE ID

l(3)rF264

Affected gene(s)
Ecol\lacZ, vn
Viability / fertility
Associated allele(s)
Ecol\lacZvn-rF264, vnrF264
Stock availability
None publicly available
Genomic Location
Chromosomal Location
3L ( 64F1-64F3 )
Sequence Location
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
Cytological location (reported)
64F1-64F3 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
P{PZ}
Size (Kb)

14.545

Component Allele(s)
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
second instar larval stage
wing disc | proximal | restricted
(Paul et al., 2013)
third instar larval stage
dorsal mesothoracic pleura primordium

Comment: notum

(Pallavi et al., 2006)
wing hinge primordium
(Pallavi et al., 2006)
wing pouch | restricted
(Pallavi et al., 2006)
anterior-posterior compartment boundary of the wing disc | restricted
(Pallavi et al., 2006)
haltere disc | restricted

Comment: presumptive hinge; NOT haltere pouch

(Pallavi et al., 2006)
Additional Information
Statement
Reference

In the wing pouch, EcollacZvn-rF264 is expressed as a broad discontinuous stripe straddling the A/P boundary in the L3 and L4 intervein region and also in the hinge and the notum, in a pattern similar to that of vn transcript. In contrast to vn transcript expression, EcollacZvn-rF264 is expressed in the presumptive hinge and notum of the haltere disc.

(Pallavi et al., 2006)
 
Marker for
Reflects expression of
vn
(Paul et al., 2013, Pallavi et al., 2006)
Reporter construct used in assay
Data on Genetic Line
Line ID

l(3)rF264

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line

t

(Gene Disruption Project members, 2001-)
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
P{PZ}vnrF264
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{PZ}vnrf264
(Grieder et al., 2009)
veinrf264-lacZ
(Pallavi et al., 2006)
vn-lacZ
(Paul et al., 2013, Grieder et al., 2009)
vnRf
(Galindo et al., 2002)
vnrF264
(Paul et al., 2013)
vnrF264-lacZ
(Paul et al., 2013)
Name Synonyms
Secondary FlyBase IDs
    References (12)