FB2026_03 , released September 17, 2026
Insertion: Dmel\P{PZ}Sema2a03474
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General Information
Symbol
Dmel\P{PZ}Sema2a03474
Species
D. melanogaster
Name
FlyBase ID
FBti0005144
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{PZ}
LINE ID

l(2)03474

Affected gene(s)
Ecol\lacZ, Sema2a
Viability / fertility
Associated allele(s)
Ecol\lacZSema2a-03474, Sema2a03474
Stock availability
None publicly available
Genomic Location
Chromosomal Location
2R ( 53C6-53C7 )
Sequence Location
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
53C6-53C7 (near gene of known cytology)
Cytological location (reported)
53C3-53C10 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
P{PZ}
Size (Kb)

14.545

Component Allele(s)
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene
Ecol\lacZ
(Kolodkin et al., 1993)
Sema2a
(Joo et al., 2013, Kolodkin et al., 1993)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
sense organ | anterior
(Kolodkin et al., 1993)
larval neuron
(Kolodkin et al., 1993)
embryonic stage 14 -- 17
hypodermal muscle cell of metathorax | ventral
(Kolodkin et al., 1993)
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
gonad
(Kolodkin et al., 1993)
embryonic stage 10
epidermis | striped
(Kolodkin et al., 1993)
embryonic stage 15 -- 17
central nervous system | restricted
(Kolodkin et al., 1993)
Additional Information
Statement
Reference

Enhancer trap expression is firstdetected at embryonic stage 10 in weak epidermal stripes. It is also foundin the CNS starting in stage 15 in a restricted set of neurons. Beginningin stage 14, EcollacZ is expressed in a single ventral thoracic muscle fiberin segment T3. Expression occurs also in embryonic gonads and anteriorsense organs.

(Kolodkin et al., 1993)
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)03474

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
P{PZ}Sema-2a03474
(FlyBase, 1992-)
P{PZ}Sema2a03474
(FlyBase, 1992-)
P{PZ}sema-II03474
(Kolodkin et al., 1993, FlyBase, 1992-)
P{PZ}semaII03474
(Kolodkin et al., 1993, FlyBase, 1992-)
Sema-2aP2
(Joo et al., 2013)
Name Synonyms
Secondary FlyBase IDs
  • FBti0003559
References (13)