FlyBase Insertion Report: Dmel\P{PZ}chn[02064]

General Information

Symbol

Dmel\P{PZ}chn⁰²⁰⁶⁴

Species

D. melanogaster

Name

FlyBase ID

FBti0005208

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)02064

Affected gene(s)

chn, Ecol\lacZ

Viability / fertility

Associated allele(s)

chn⁰²⁰⁶⁴, Ecol\lacZ^chn-02064

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 51E2 )

Sequence Location

2R:15,129,586..15,129,586 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:15,129,586..15,129,586 [+]

Orientation

Cytological location (computed by FlyBase)

51E2 (inferred by FlyBase from sequence location)

Cytological location (reported)

51E1-51E2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

G00580

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

chn

(Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Russell et al., 1998, Vosshall, 1998.8.10, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

chn⁰²⁰⁶⁴

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^chn-02064

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval pharynx

(Jackle and Janning, 1998.8.10)

presumptive embryonic/larval peripheral nervous system

(Jackle and Janning, 1998.8.10)

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

larval brain

(Meister and Braun, 1995.10)

embryonic/larval midgut

(Meister and Braun, 1995.10)

Additional Information

Statement

Reference

In embryo, expression in PNS and pharynx not certain.

(Jackle and Janning, 1998.8.10)

In wandering third instar, low level of expression in larval brain, imaginal discs, and larval midgut. (Not specified which imaginal discs.)

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

chn

(Meister and Braun, 1995.10)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)02064

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11172

cn¹ P{PZ}chn⁰²⁰⁶⁴/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

P{PZ}chn⁰²⁰⁶⁴

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}l(2)02064⁰²⁰⁶⁴

(FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)02064⁰²⁰⁶⁴

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000287

References (14)

Report Sections

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