FlyBase Insertion Report: Dmel\P{PZ}wal[02516]

General Information

Symbol

Dmel\P{PZ}wal⁰²⁵¹⁶

Species

D. melanogaster

Name

FlyBase ID

FBti0005218

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)02516

Affected gene(s)

Ecol\lacZ, wal

Viability / fertility

Associated allele(s)

Ecol\lacZ^wal-02516, wal⁰²⁵¹⁶

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 48C1 )

Sequence Location

2R:11,690,833..11,690,833 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:11,690,833..11,690,833 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

48C1 (inferred by FlyBase from sequence location)

Cytological location (reported)

48C1-48C2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Genomic location previously reported as 2R:7578339..7578347 .

Sequence Data

Flanking sequence

AQ025595

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

wal

(Yang et al., 2013, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^wal-02516

Phenotypic Class Phenotype Manifest in Detailed Description

wal⁰²⁵¹⁶

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult optic lobe

(Vosshall, 1998.8.10)

adult brain | restricted

(Vosshall, 1998.8.10)

adult central complex

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In the adult brain, expressed in the central brain and the optic lobe.

(Vosshall, 1998.8.10)

In wandering third instar larva, no β-gal staining observed.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

wal

(Meister and Braun, 1995.10)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)02516

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11198

cn¹ P{PZ}wal⁰²⁵¹⁶/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Location 2R:7206740-7206741 confirmed by FlyBase alignment of dbGSS accession AQ025595 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)

8 bp insertion associated host repeat

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P1198

(Meister and Braun, 1995.10)

P{PZ}l(2)02516⁰²⁵¹⁶

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}wal⁰²⁵¹⁶

(FlyBase, 2005, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)02516⁰²⁵¹⁶

(FlyBase, 1992-)

wal⁰²⁵¹⁶

(Yang et al., 2013)

Name Synonyms

Secondary FlyBase IDs

FBti0000297

References (15)

Report Sections

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