FlyBase Insertion Report: Dmel\P{PZ}Nup214[10444]

General Information

Symbol

Dmel\P{PZ}Nup214¹⁰⁴⁴⁴

Species

D. melanogaster

Name

FlyBase ID

FBti0005348

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)10444

Affected gene(s)

Ecol\lacZ, Nup214

Viability / fertility

Associated allele(s)

Ecol\lacZ^Nup214-10444, Nup214¹⁰⁴⁴⁴

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 59B6 )

Sequence Location

2R:22,924,533..22,924,533 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:22,924,533..22,924,533 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

59B6 (inferred by FlyBase from sequence location)

Cytological location (reported)

59B6-59B7 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ025675

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Csink et al., 2002, Martin et al., 2001, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Nup214

(Chavan et al., 2024, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^Nup214-10444

Phenotypic Class Phenotype Manifest in Detailed Description

Nup214¹⁰⁴⁴⁴

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

adult stage

adult brain | restricted

(Vosshall, 1998.8.10)

adult central complex | restricted

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In the adult, expressed in a discrete region of the central brain.

(Vosshall, 1998.8.10)

In wandering third instar larva, expression in imaginal discs (which discs not specified).

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)10444

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

12369

P{PZ}Nup214¹⁰⁴⁴⁴ cn¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Location 2R:18432133-18432134 confirmed by FlyBase alignment of dbGSS accession AQ025675 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

10444

(Csink et al., 2002)

P2369

(Meister and Braun, 1995.10)

P{PZ}Nup214¹⁰⁴⁴⁴

(FlyBase, 2005, FlyBase, 1992-)

P{PZ}l(2)10444¹⁰⁴⁴⁴

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)10444¹⁰⁴⁴⁴

(Martin et al., 2001, FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000434

References (12)

Report Sections

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