FlyBase Insertion Report: Dmel\P{PZ}not[02069]

General Information

Symbol

Dmel\P{PZ}not⁰²⁰⁶⁹

Species

D. melanogaster

Name

FlyBase ID

FBti0005444

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)02069

Affected gene(s)

Ecol\lacZ, not

Viability / fertility

Associated allele(s)

Ecol\lacZ^not-02069, not⁰²⁰⁶⁹

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 75D4 )

Sequence Location

3L:18,621,449..18,621,449 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3L:18,621,449..18,621,449 [+]

Orientation

Cytological location (computed by FlyBase)

75D4 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Previously reported in Fly

Base as 3L:18614549..18614549 based on accession GB:G00744.

The insertion was reported to be in the first exon of not, 538bp downstream of the start codon. The mapping was based on the use of a start codon in the publication that maps 717 bases upstream of the currently annotated start codon and upstream of the transcription start site in FlyBase.

(Poeck et al., 2001)

Genomic location previously reported as 3L:18614770..18614770 .

(Poeck et al., 2001)

Sequence Data

Flanking sequence

G00744

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545