Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
l(2)k16510
Location 2L:249373-249374 confirmed by FlyBase alignment of dbGSS accession AQ026108 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
The sites of insertion of P{lacW}k10237 and P{lacW}l(2)k16510k16510 are 7bp apart.
The lethality of the "l(2)k16510" chromosome (caused by l(2)k16510k16510) is separable from the P{lacW}k16510 insertion.The sites of insertion of P{lacW}k10237 and P{lacW}k16510 are 7bp apart.
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).