FlyBase Insertion Report: Dmel\P{lacW}k08617

General Information

Symbol

Dmel\P{lacW}k08617

Species

D. melanogaster

Name

FlyBase ID

FBti0006624

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{lacW}

LINE ID

l(2)k08617

Affected gene(s)

bowl, Ecol\lacZ

Viability / fertility

Associated allele(s)

Ecol\lacZ^k08617

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 24C3 )

Sequence Location

2L:3,771,702..3,771,702 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{lacW}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w^+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:3,771,702..3,771,702 [-]

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

24C3 (inferred by FlyBase from sequence location)

Cytological location (reported)

24C1-24C2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ025865

(FlyBase, 1992-)

Inserted Element

Construct

P{lacW}

Size (Kb)

10.691

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

bowl

(Hao et al., 2003, BDGP Project Members, 1994-1999)

Ecol\lacZ

(Hao et al., 2003, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^k08617

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

leg disc | restricted

(Hao et al., 2003)

pupal stage

leg | segmentally repeated

(Hao et al., 2003)

Additional Information

Statement

Reference

Larval expression is observed in the center of the leg discs. Pupal expression is observed in a broad stripe in each developing leg segment, including tarsal segments 1-4.

(Hao et al., 2003)

Marker for

Reflects expression of

bowl

(Hao et al., 2003)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)k08617

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

111243

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}k08617 P{neoFRT}40A/CyO y⁺

External Crossreferences and Linkouts ( 0 )

Comments

Location 2L:3771701-3771702 determined by FlyBase alignment of dbGSS accession AQ025865 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

P{lacW}bowl^k08617

(FlyBase, 1992-)

P{lacW}k08617

(FlyBase, 1992-)

P{lacW}l(2)k08617^k08617

(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)

bowl-LacZ

(Hao et al., 2003)

bowl-lacZ

(Hao et al., 2003)

p^K08617

(Dearborn et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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