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FB2026_02
,
released June 18, 2026
Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w+mC and the mini-yellow marker y+mDint2, and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
The EY03976 insertion stock was previously reported to contain two insertions on 3L; one at 3L:3460603..3460603 (release 5 coordinates) based on 5' flanking sequence recovered by inverse PCR and one at and 3L:6970284..6970284 (release 5 coordinates) based on 3' flanking sequence. However, the 5' flanking sequence at 3L:3460603..3460603 was most likely a cross-contamination artifact as it was the same sequence as that of a sample in an adjacent well on the 96 well plate. It is therefore likely that the EY03976 insertion stock contains only a single insertion at 3L:6970284..6970284 . This single insertion is represented by P{EPgy2}G-iα65AEY03976b.
Location 3L:6951130-6951131 determined by FlyBase alignment of dbGSS accession CC060286 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.