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General Information
Name
TI_set_P{EPgy2}.EY
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000513
Vector
    Title
    A set of P-element construct P{EPgy2} insertions.
    Accessions
      Overview
      Description

      A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w+mC and the mini-yellow marker y+mDint2, and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.

      Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.

      Available from
      Bloomington Drosophila Stock Center
      Not all insertions in this dataset are available as extant stocks.
      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Transgenic Construct used
      Protocol

      The P{EPgy2} construct is similar to P{EP}, but incorporates a modification that allows expression driven by the Scer\GAL4 regulatory sequences to occur in the female germline (seeFBrf0128569). Mobilization of the P-element occurred in animals carrying P{EPgy2} on a second chromosome CyO balancer chromosome and P{Δ2-3}99B, a stable source of transposase, on a marked third chromosome. Genomic locations of new insertions were determined by assessment of flanking sequence by high-throughput methods and balanced stocks were created.

      Markers in stocks (as originally submitted to the BDSC):y1 w67c23.

      Mode of Assay
      Data analysis

      Flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence; 2338 lines were selected for inclusion in the BDGP Gene Disruption Project 2004 collection.

      An additional 11,830 P{EPgy2} insertion lines were generated and 9585 were mapped to unique sites; 1193 were selected for addition to the BDGP Gene Disruption Project collection.

      Comments
      Associated Data
      Size
      Associated features
      3,576 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      GDP_insertions_P{EPgy2}.EY
      TI_set_P{EPgy2}.EY
      Name Synonyms
      A set of P-element construct P{EPgy2} insertions.
      Secondary FlyBase IDs
        References (6)