FlyBase Insertion Report: Dmel\PBac{681.P.FSVS-1}dpy[CPTI001769]

General Information

Symbol

Dmel\PBac{681.P.FSVS-1}dpy^CPTI001769

Species

D. melanogaster

Name

FlyBase ID

FBti0143891

Feature type

transposable_element_insertion_site

Also Known As

dpy-YFP, dpy::YFP

Description

Inserted Element

PBac{681.P.FSVS-1}

LINE ID

Affected gene(s)

Avic\GFP, dpy

Viability / fertility

Associated allele(s)

Avic\GFP^{dpy-CPTI001769}, dpy^CPTI001769

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 25A1 )

Sequence Location

2L:4,551,618..4,551,618 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_PBac{P.SVS-0,1,2}.CPTI

Description

A set of transgenic insertion stocks derived by TE mobilization using one of the hybrid Tni\piggyBac-P-element protein-trap constructs PBac{566.P.SVS-1}, PBac{602.P.SVS-1}, PBac{681.P.FSVS-1}, PBac{754.P.FSVS-0}, PBac{802.P.SVS-2}, PBac{803.P.SVS-0}, or PBac{810.P.FSVS-2}. The constructs carry a w^+mC mini-white visible marker and a Strep-tagged Avic\GFP vital fluorescent protein-trap marker (using the Venus YFP variant of GFP); P-element termini are nested inside PBac termini. The frame used for the splice acceptor and splice donor consensus sequences relative to the Avic\GFP sequence is indicated by 0, 1, or 2 in the construct symbol.

(FlyProt project., 2009.11)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:4,551,618..4,551,618 [+]

(FlyProt project., 2011.8.8)

Orientation

(FlyProt project., 2011.8.8)

Cytological location (computed by FlyBase)

25A1 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

PBac{681.P.FSVS-1}

(FlyProt project., 2011.8.8)

Size (Kb)

12.209

(FlyProt project., 2009.11)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

Avic\GFP

(FlyProt project., 2011.8.8)

dpy

(Chu and Hayashi, 2021, FlyProt project., 2011.8.8, Rees et al., 2011)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Avic\GFP^{dpy-CPTI001769}

Phenotypic Class Phenotype Manifest in Detailed Description

dpy^CPTI001769

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

imaginal disc epithelial cell

(Ray et al., 2015)

(Ray et al., 2015)

(Ray et al., 2015)

(Ray et al., 2015)

leg | distal

(Ray et al., 2015)

antenna | distal

(Ray et al., 2015)

adult stage

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

Additional Information

Statement

Reference

A protein trap insertion using a yellow fluorescent protein (YFP) tag was used to observe dpy protein expression in the larval imaginal discs and in pupae. In the imaginal discs, dpy is found in a dense meshwork covering the apical surface of the epithelium. It is more restricted in the pupal wing. At the onset of hinge contraction (18 hr APF), it is restricted to the wing margin and along the trajectories of wing veins L3 and L5. It is also observed at the extreme distal tips of the pupal legs and antennae. As tissue contraction proceeds, dpy expression occurs uniformly over the apical surface of epithelium.

(Ray et al., 2015)

Marker for

Reflects expression of

dpy

(Ray et al., 2015, Knowles-Barley, 2011.8.24)

Reporter construct used in assay

Avic\GFP^{dpy-CPTI001769}

(Knowles-Barley, 2011.8.24)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

115238

w¹¹¹⁸; PBac{681.P.FSVS-1}dpy^CPTI001769

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

1769

(Rees et al., 2011)

CPTI-001769

(Drees et al., 2023, FlyProt project., 2011.8.8)

CPTI001769

(Drees et al., 2019)

Dp-YFP

(Diaz-de-la-Loza et al., 2020)

Dpy-YFP

(Woodward et al., 2025, Ríos-Barrera et al., 2017)

Dpy::YFP

(Tsuboi et al., 2023, Ayukawa et al., 2022)

Dpy::eYFP

(Drees et al., 2023)

Dumpy-YFP

(Diaz-de-la-Loza et al., 2020)

Dumpy::eYFP

(Drees et al., 2023)

Dumpy:YFP

(Smith et al., 2020)

PBac{681.P.FSVS-1}dp^CPTI001769

PBac{681.P.FSVS-1}dpy^CPTI001769

dp-FYP

(Ray et al., 2015)

dpy-YFP

(Chu and Hayashi, 2021)

dpy::YFP

(Drees et al., 2019)

dumpy::YFP

(Drees et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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