FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Insertion: Dmel\Mi{PT-GFSTF.0}rheaMI00296-GFSTF.0
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General Information
Symbol
Dmel\Mi{PT-GFSTF.0}rheaMI00296-GFSTF.0
Species
D. melanogaster
Name
FlyBase ID
FBti0147808
Feature type
transposable_element_insertion_site
Description
Inserted Element
Mi{PT-GFSTF.0}
LINE ID

MI00296-GFSTF.0

Affected gene(s)
Avic\GFP, lncRNA:CR43910, rhea
Viability / fertility
Associated allele(s)
lncRNA:CR43910MI00296-GFSTF.0-X, rheaMI00296-GFSTF.0
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
3L ( 66D6 )
Sequence Location
3L:8,569,653..8,569,653 [-]
Target / Docking site
Mi{MIC}rheaMI00296
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_Mi{PT-GFSTF.0,1,2}.GDP
Description

A set of transgenic insertion stocks containing a protein trap cassette, derived from Mi{MIC} insertions in coding introns using Recombinase-Mediated Cassette Exchange (RMCE); see http://flypush.imgen.bcm.tmc.edu/pscreen/technique.html. The protein trap cassette contains a EGFP-FlAsH-StrepII-TEV-3xFlag ("GFSTF") tag that is flanked on either side with a 4X(GlyGlySer) flexible linker; the mini-yellow marker is no longer present. Each insertion line contains one of three versions of the cassette (Mi{PT-GFSTF.0}, Mi{PT-GFSTF.1} or Mi{PT-GFSTF.2}), depending on the reading frame required to generate an in-frame fusion protein with the gene in which the insertion is located. This system also allows UAS/GAL4-based knockdown strategies targeting GFP-sequence containing mRNA (RNAi against GFP) or GFP fusion protein (deGradFP).

(Nagarkar-Jaiswal et al., 2015)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:8,569,653..8,569,653 [-]
(Gene Disruption Project members, 2015-)
Orientation

-

(Gene Disruption Project members, 2015-)
Cytological location (computed by FlyBase)
66D6 (inferred by FlyBase from sequence location)
Cytological location (reported)
66D6_r6 (reported as inferred from sequence location)
(Gene Disruption Project members, 2015-)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
Mi{PT-GFSTF.0}
(Venken et al., 2011)
Size (Kb)
Component Allele(s)
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

MI00296-GFSTF.0

(Gene Disruption Project members, 2015-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Linkouts
GDP-RMCE
  • RM00364
Comments
Comments

The attP cassette of the Mi{MIC} element has been replaced with the attB protein-trap cassette from the pBS-KS-attB1-2-PT-SA-SD-0-EGFP-FlAsH-StrepII-TEV-3xFlag plasmid by phiC31-mediated recombination. The attB protein-trap cassette contains a multi-tag cassette consisting of the EGFP version of Avic\GFP, T:Zzzz\TC, T:Zzzz\StrepII, T:Zzzz\TEV.CS and 3xT:Zzzz\FLAG.

(Venken et al., 2011)
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Mi{PT-GFSTF.0}rheaMI00296-GFSTF.0
(Gene Disruption Project members, 2015-)
rhea-EGFP
(Kroeger et al., 2024)
rheaMI00296-GFP
(Ambrosini et al., 2019)
Name Synonyms
Secondary FlyBase IDs
    References (4)