FB2026_03 , released September 17, 2026
Insertion: Dmel\P{GT1}CkIIα-i3BG00973b
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General Information
Symbol
Dmel\P{GT1}CkIIα-i3BG00973b
Species
D. melanogaster
Name
FlyBase ID
FBti0148455
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{GT1}
LINE ID
Affected gene(s)
CkIIα-i3, Scer\GAL4
Viability / fertility
Associated allele(s)
CkIIα-i3BG00973b, Scer\GAL4CkIIα-i3-BG00973b
Stock availability
None publicly available
Genomic Location
Chromosomal Location
3L ( 61C8 )
Sequence Location
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GT1}.BG
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GT1}. The P{GT1} construct is described as a dual-tagged gene trap (FBrf0132344):it carries (1) w+mGT1 mini-white marker, designed to confer a pigmented eye phenotype only if the construct is inserted within a host gene, and (2) a promoter-less Scer\GAL4 gene which is expressed if it is produced as a fusion with the mRNA produced by the host gene. The construct also carries a Tn\neoR selectable marker (FBrf0132344).

(Bellen et al., 2004)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
61C8 (near gene of known cytology)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
P{GT1}
(Magwire et al., 2010)
Size (Kb)

8.447

(Lukacsovich and Asztalos, 1999.7.15)
Component Allele(s)
w+mGT
Molecular map
Affected Gene(s)
Insertion may affect gene
CkIIα-i3
(Tunstall et al., 2012)
Scer\GAL4
(Tunstall et al., 2012)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
olfactory sensory organ | restricted | subset
(Tunstall et al., 2012)
Additional Information
Statement
Reference

ScerGAL4CkIIα-i3-BG00973b drives expression in neurons associated with olfactory sensory organs of the second and third antennal segments, maxillary palp, mouthparts, and legs; brain expression is not observed.

(Tunstall et al., 2012)
 
Marker for
Reflects expression of
CkIIα-i3
(Tunstall et al., 2012)
Reporter construct used in assay
Mmus\Cd8aUAS.cLa.EGFP
(Tunstall et al., 2012)
Data on Genetic Line
Line ID
Origin as a multiple insertion line

y

(Tunstall et al., 2012)
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Southern analysis indicates that there are multiple P{GT1} insertions in this line; 3' RACE supports insertion into CkIIα-i3 gene.

(Tunstall et al., 2012)

Southern blots indicate the presence of two insertions in the original BG00973 line. Further analysis by PCR confirms the presence of two insertions: P{GT1}BG00973a and P{GT1}CkIIα-i3BG00973b.

(Tunstall et al., 2012)
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
BG00973
(Tunstall et al., 2012)
P{GT1}CkIIα-i3BG00973
P{GT1}CkIIα-i3BG00973b
Name Synonyms
Secondary FlyBase IDs
    References (3)