The p65(AD)::CRY2 hemidriver contains the transcriptional activation domain from the Homo sapiens 'p65' gene (RELA, HGNC:9955) fused via a flexible linker to the the Arabidopsis thaliana CRY2 gene (TAIR:28374). p65(AD)::CRY2 forms one half of a light-regulated split lexA system when combined in vivo with a hemidriver containing a DNA-binding domain fused to an N-terminal portion (amino acids 1-171) of Arabidopsis thaliana CIB1 (TAIR:130033). Upon illumination with blue light, CRY2 undergoes a conformational change and can bind the CIBN peptide, bringing the two hemidrivers together and reconstituting a functional transcriptional activator that can drive transcription of a downstream gene of interest. Since transcription is only activated at the intersection of the expression patterns of the two hemidrivers and upon activation by light, this system can be used to drive expression in a restricted pattern (FBrf0229642). The DNA-binding specificity of the reconstituted transcriptional activator will depend upon the DNA-binding domain present in the CIBN-domain containing hemidriver.