deGradALFA is an engineered F-box protein that targets Tag:ALFA-tagged proteins for degradation via the ubiquitin pathway. deGradALFA contains the N-terminal part of D. melanogaster slmb (including the F-box domain), fused to NbALFA, an engineered single-chain variable fragment antibody that binds the Tag:ALFA epitope. Upon expression of deGradALFA, target proteins tagged with Tag:ALFA are specifically polyubiquitinated and rapidly degraded by the proteasome (FBrf0260618).