FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{HBΔ-194}
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General Information
Symbol
P{HBΔ-194}
FlyBase ID
FBtp0000178
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZHsp70Bb.-194
Size
16.898
Expression Data
lacZ reporter
Associated insertion(s)
3 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
ry+t7.2
Component allele Ecol\lacZHsp70Bb.-194
Transgenic product class
Component
Use(s)
Encoded product / tool
lacZ
Description

Hsp70Bb promoter sequences from 194bp upstream of the transcription start site and some Hsp70Bb coding sequence is fused to lacZ.

(Lis et al., 1983)

Hsp70 promoter sequences from position -194 to +1250 are fused in frame to lacZ.

(Vincent et al., 1990)
Sequence Data
Sequence (FB)
FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size

16.898

Left end

P5\'

(Simon et al., 1985)
Right end

P3\'

(Simon et al., 1985)
 
Segments
Num
Orientation
Symbol
1
+
Car1_P5'(P5',R1)
2
+
001111
3
-
Hsp70::lacZ(ep19.1)
4
+
001110
5
+
Car20_P3'(H3,P3')
Reference
(FlyBase, 1992-)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
organism | ubiquitous
(Papaconstantinou et al., 2005)
larval stage
organism | ubiquitous
(Lis et al., 1983)
adult stage
organism | ubiquitous
(Lis et al., 1983)
adult stage & oogenesis
egg chamber
(Lis et al., 1983)
Additional Information
Statement
Reference

Subsequent to heat-shock, high levels of expression observed in most tissues. Higher levels observed in early egg chambers relative to late egg chambers. Quantitation hindered by variable permeability of different structures and tissues, affecting uptake of the X-gal substrate. Without heat-shock, expression cannot be distinguished from background levels.

(Lis et al., 1983)
 
Marker for
Reflects expression of
Hsp70Bb
(Papaconstantinou et al., 2005)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{HBΔ-194}
(Simon et al., 1985)
descendant(s)
Comments

construct_comment: One of a series of constructs used to characterize promoter sequences and sequences required for puffing within Hsp70Bb. Produces new heat-inducible puff at cytological site of insertion.

(Simon et al., 1985)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
HBΔ-194
(Simon et al., 1985)
P{HBΔ-194}
(FlyBase, 1996-, FlyBase, 1992-)
Secondary FlyBase IDs
    References (8)