FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Recombinant Construct: H{Lw2}
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General Information
Symbol
H{Lw2}
FlyBase ID
FBtp0000910
Feature type
transgenic_transposable_element
Component Allele(s)
Size
13.232
Expression Data
Associated insertion(s)
53 available
Molecular map
Description and Uses
Description

H{Lw2} is an enhancer trap construct that encodes a lacZ reporter. The enhancer trap sequence is at the 3' end of the construct and consists of weak promoter sequence from the Hsp70 gene (-50 to +271, includes the TATA box but lacks heat shock-inducible sequences, also encodes the first 7 amino acids of Hsp70) fused in-frame upstream of the lacZ coding sequence.

(Smith et al., 1993)
Construct components
Component
Use(s)
Element type
Encoded product / tool
lacZ
Marker(s)
w+mC
Sequence Data
Sequence (FB)
FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size

13.232

Left end

H5\'

(Smith et al., 1993)
Right end

H3\'

(Smith et al., 1993)
 
Segments
Num
Orientation
Symbol
1
+
hobo5'(H5',Xb)
2
+
XbaI/SpeI
3
+
w+mC4.1(Sp)
4
-
001172
5
-
pHSS7(N,R1)
6
-
001115a
7
-
Hsp70Bb::lacZ_4.1
8
+
KpnIor/EcoRIef
9
+
hobo3'(R1,H3')
Reference
(Smith et al., 1993)
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pH{Lw2}
(Smith et al., 1993)
descendant(s)
Comments

sequence comment: CAUTION: Orientation of segment M13ori_181 not known; arbitrarily presented in positive orientation in progenitor (pP{FZ}).Size of segment Hsp70Ab+0.5(r) is an estimation.May be additional errors.

(Smith et al., 1993)

report comment: The enhancer trap allele, lacZHZ50a, is theoretically identical to the allele found in pP{HZ50PL}, however only the 5\' end is actually derived from pP{HZ50PL}; the 3\' end is derived from pP{FZ}.

(Smith et al., 1993)

mapping_comment: Although segment 11, Hsp70Bb::lacZ_4.1 , is the same as that found in pP{HZ50PL}, only the 5\' end (adjacent to hobo 3\' end) is actually derived from pP{HZ50PL}. The 3\' end (adjacent to neoR) is derived from pP{FZ}; fusion at Bsu36I site at 5\' end of lacZ segment.

(Smith et al., 1993)

construct_comment: Useful sites for plasmid rescue (Brian Calvi, personal communication to FlyBase): cloning to left, NdeI, BamHI, MluI; cloning to right, ApaI, NsiI, HindIII. Resulting clones are kanamycin resistant.

(Smith et al., 1993)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
H[pHLw2]
(Smith et al., 1993)
H{Lw2}
(FlyBase, 1996-)
H{lacZ}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (7)