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General Information
Symbol
H{Lw2}
FlyBase ID
FBtp0000910
Feature type
transgenic_transposable_element
Component Allele(s)
Size
13.232
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Encoded product / tool
Marker(s)
Description

H{Lw2} is an enhancer trap construct that encodes a lacZ reporter. The enhancer trap sequence is at the 3' end of the construct and consists of weak promoter sequence from the Hsp70 gene (-50 to +271, includes the TATA box but lacks heat shock-inducible sequences, also encodes the first 7 amino acids of Hsp70) fused in-frame upstream of the lacZ coding sequence.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size

13.232

Left end
Right end
 
Features
CV term
Qualifiers and info
Reference
bacterial
selection
partial
rescue
selectable
marker
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
descendant(s)
Comments

sequence comment: CAUTION: Orientation of segment M13ori_181 not known; arbitrarily presented in positive orientation in progenitor (pP{FZ}).Size of segment Hsp70Ab+0.5(r) is an estimation.May be additional errors.

report comment: The enhancer trap allele, lacZHZ50a, is theoretically identical to the allele found in pP{HZ50PL}, however only the 5\' end is actually derived from pP{HZ50PL}; the 3\' end is derived from pP{FZ}.

mapping_comment: Although segment 11, Hsp70Bb::lacZ_4.1 , is the same as that found in pP{HZ50PL}, only the 5\' end (adjacent to hobo 3\' end) is actually derived from pP{HZ50PL}. The 3\' end (adjacent to neoR) is derived from pP{FZ}; fusion at Bsu36I site at 5\' end of lacZ segment.

construct_comment: Useful sites for plasmid rescue (Brian Calvi, personal communication to FlyBase): cloning to left, NdeI, BamHI, MluI; cloning to right, ApaI, NsiI, HindIII. Resulting clones are kanamycin resistant.

Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Secondary FlyBase IDs
    References (6)