construct_comment: One of two vectors used by Berkeley Drosophila Genome Project.Reference: Pierce et al., 1992, Proc. Natl. Acad. Sci. USA 89:2056-2060.Insertion in BamHI cloning site prevents expression of sacBII, allowing selection in medium containing high levels of sucrose.PLEASE NOTE: Sequence available for pSacBII, which appears to be the same vector without the Ad10 stuffer fragment (see Descendants).
construct_comment: One of two vectors used by Berkeley Drosophila Genome Project.Reference: Pierce et al., 1992, Proc. Natl. Acad. Sci. USA 89:2056-2060.Insertion in BamHI cloning site prevents expression of sacBII, allowing selection in medium containing high levels of sucrose.PLEASE NOTE: Sequence available for pSacBII, which appears to be the same vector without the Ad10 stuffer fragment (see Descendants).
report comment: Insertion of sacBII_cassette between the BamHI and SalI sites in the tetR gene of P1(pNS582-tet14_Ad10).