FB2026_02 , released June 18, 2026
Recombinant Construct: P{FRT(iwoff)}
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General Information
Symbol
P{FRT(iwoff)}
FlyBase ID
FBtp0001600
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

P{FRT(iwoff)} contains a non-functional w minigene consisting of a 5' portion of w, an FRT site, the 3' portion of w (in reverse orientation to the 5' portion) and a second FRT site (in the opposite orientation to the first one). The P{FRT(iwoff)} transgene is generated in vivo by FLP-mediated inversion of the sequence flanked by FRT sites in P{FRT(iwon)}. This process is reversible; FLP can be used to reinvert the FRT flanked sequence in P{FRT(iwoff)} to regenerate P{FRT(iwon)} (which contains the functional w+mW.FRT.hs marker).

(Ahmad and Golic, 1996)
Construct components
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
P{FRT(iwon)}
(Ahmad and Golic, 1996)
descendant(s)
Comments

construct_comment: Derived from P{FRT(iwon)} by internal recombination event catalyzed by FLP1 recombinase; reversible reaction.

(Ahmad and Golic, 1996)

report comment: See report for Scer\FRT.

(Ahmad and Golic, 1996)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P[iw]off
(Ahmad and Golic, 1996)
P{FRT(iwoff)}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)